Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9726 and 9727 are separated by 1009 nucleotides9727 and 9728 are separated by 319 nucleotides9728 and 9729 are separated by 613 nucleotides9729 and 9730 are separated by 935 nucleotides mRNA_1358: 9726 - K01648 ACLY ATP citrate (pro-S)-lyase, at 80,345 to 84,761 9726 mRNA_1359: 9727 - K06018 PPN1 endopolyphosphatase, at 85,771 to 88,302 9727 mRNA_1360: 9728 - K01054 MGLL acylglycerol lipase, at 88,622 to 90,157 9728 mRNA_1361: 9729 - Hypothetical Protein, at 90,771 to 91,579 9729 mRNA_1362: 9730 - K00507 SCD, desC stearoyl-CoA desaturase (Delta-9 desaturase), at 92,515 to 94,818 9730
Group Condition mRNA_1358 mRNA_1359 mRNA_1360 mRNA_1361 mRNA_1362
9726 9727 9728 9729 9730
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.9 -0.2 -0.3 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. -1.3 +0.0 +0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -1.0 -0.4 +0.2 N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. -1.4 -0.3 +0.6 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.5 +0.2 -0.7 N.D.
growth on oleic acid YNB Oleic Acid N.D. -0.2 -0.6 -0.2 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.6 -0.1 -0.1 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.0 -0.8 -0.0 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. -0.4 -0.1 -0.2 N.D.
growth on oleic acid YNB Glucose N.D. -0.3 -0.1 -0.2 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.4 +0.2 -0.2 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. -0.9 +0.0 +0.5 N.D.
auxotrophy Dropout Complete N.D. -0.9 -0.1 +0.7 N.D.
growth on ricinoleic acid YNB Glucose N.D. -0.4 -0.1 +0.2 N.D.
growth on ypd YPD N.D. -0.2 +0.2 -0.2 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. -0.5 -0.1 +0.5 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.1 +0.1 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.3 -0.0 -0.0 N.D.
auxotrophy YNB Glucose N.D. -0.5 +0.0 +0.7 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.5 +0.3 +0.4 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +1.1 +0.7 +0.2 N.D.
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