Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9748 and 9749 are separated by 172 nucleotides9749 and 9750 are separated by 194 nucleotides9750 and 9751 are separated by 442 nucleotides9751 and 9752 are separated by 185 nucleotides mRNA_1380: 9748 - K02155 ATPeV0C, ATP6L V-type H+-transporting ATPase 16kDa proteolipid subunit, at 136,828 to 138,135 9748 mRNA_1381: 9749 - Hypothetical Protein, at 138,308 to 139,656 9749 mRNA_1382: 9750 - K11805 WDR68, HAN11 WD repeat-containing protein 68, at 139,851 to 141,572 9750 mRNA_1383: 9751 - HMMPfam-Nse4 C-terminal-PF08743,HMMPfam-Binding domain of Nse4/EID3 to Nse3-MAGE-PF15412, at 142,015 to 143,615 9751 mRNA_1384: 9752 - KOG4472 Glycolipid 2-alpha-mannosyltransferase (alpha-1,2-mannosyltransferase), at 143,801 to 145,438 9752
Group Condition mRNA_1380 mRNA_1381 mRNA_1382 mRNA_1383 mRNA_1384
9748 9749 9750 9751 9752
ypd buoyancy 40 hrs No Buoyancy Separation N.D. +0.6 -0.2 N.D. -1.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.1 -0.2 N.D. -0.3
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. -0.1 +0.0 N.D. -0.3
growth on ypd YPD N.D. -0.2 +0.4 N.D. -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. -0.2 -0.0 N.D. +0.1
growth on oleic acid YNB Oleic Acid N.D. -0.1 -0.0 N.D. +0.1
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.0 -0.0 N.D. +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. -0.0 +1.0 N.D. -0.8
growth on oleic acid YNB Glucose N.D. -0.0 +0.1 N.D. +0.2
auxotrophy YNB Glucose 75mM Methionine N.D. +0.1 +0.1 N.D. +0.1
ypd buoyancy 40 hrs Low Buoyancy N.D. -0.1 +0.2 N.D. +0.2
ypd buoyancy 40 hrs High Buoyancy N.D. +0.0 +0.1 N.D. +0.3
ypd buoyancy 40 hrs Median Buoyancy N.D. -0.1 +0.2 N.D. +0.4
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. -0.1 +0.1 N.D. +0.7
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.1 -0.1 N.D. +0.9
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.2 +0.1 N.D. +0.8
auxotrophy Dropout Complete N.D. -0.0 +0.4 N.D. +0.4
auxotrophy YNB Glucose 75mM Arginine N.D. +0.1 +0.1 N.D. +0.7
growth on ricinoleic acid YNB Glucose N.D. +0.1 +0.3 N.D. +0.6
auxotrophy YNB Glucose N.D. +0.1 +0.3 N.D. +0.7
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.0 +0.6 N.D. +0.9
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