Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9749 and 9750 are separated by 194 nucleotides9750 and 9751 are separated by 442 nucleotides9751 and 9752 are separated by 185 nucleotides9752 and 9753 are separated by 453 nucleotides mRNA_1381: 9749 - Hypothetical Protein, at 138,308 to 139,656 9749 mRNA_1382: 9750 - K11805 WDR68, HAN11 WD repeat-containing protein 68, at 139,851 to 141,572 9750 mRNA_1383: 9751 - HMMPfam-Nse4 C-terminal-PF08743,HMMPfam-Binding domain of Nse4/EID3 to Nse3-MAGE-PF15412, at 142,015 to 143,615 9751 mRNA_1384: 9752 - KOG4472 Glycolipid 2-alpha-mannosyltransferase (alpha-1,2-mannosyltransferase), at 143,801 to 145,438 9752 mRNA_1385: 9753 - K06966 K06966 uncharacterized protein, at 145,892 to 146,941 9753
Group Condition mRNA_1381 mRNA_1382 mRNA_1383 mRNA_1384 mRNA_1385
9749 9750 9751 9752 9753
ypd buoyancy 40 hrs No Buoyancy Separation +0.6 -0.2 N.D. -1.1 -0.6
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.1 +0.0 N.D. -0.3 +0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 -0.0 N.D. +0.1 +0.1
facs on lipid accumulation Sorted High BODIPY Gate +0.0 -0.0 N.D. +0.1 +0.0
growth on ypd YPD -0.2 +0.4 N.D. -0.4 +0.2
growth on oleic acid YNB Oleic Acid -0.1 -0.0 N.D. +0.1 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 -0.2 N.D. -0.3 +0.6
growth on oleic acid YNB Glucose -0.0 +0.1 N.D. +0.2 +0.1
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 +1.0 N.D. -0.8 +0.3
facs on lipid accumulation Sorted No BODIPY Gate -0.2 +0.1 N.D. +0.8 -0.2
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.1 N.D. +0.3 +0.2
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.2 N.D. +0.4 +0.1
ypd buoyancy 40 hrs Low Buoyancy -0.1 +0.2 N.D. +0.2 +0.3
auxotrophy YNB Glucose 75mM Methionine +0.1 +0.1 N.D. +0.1 +0.5
auxotrophy Dropout Complete -0.0 +0.4 N.D. +0.4 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.1 +0.1 N.D. +0.7 +0.3
growth on ricinoleic acid YNB Glucose +0.1 +0.3 N.D. +0.6 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 -0.1 N.D. +0.9 +0.5
auxotrophy YNB Glucose +0.1 +0.3 N.D. +0.7 +0.2
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.1 N.D. +0.7 +0.5
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 +0.6 N.D. +0.9 +0.0
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