Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9756 and 9757 are separated by 1043 nucleotides9757 and 9758 are separated by 406 nucleotides9758 and 9759 are separated by 192 nucleotides9759 and 9760 are separated by 664 nucleotides mRNA_1388: 9756 - K00698 CHS1 chitin synthase, at 150,149 to 154,291 9756 mRNA_1389: 9757 - Hypothetical Protein, at 155,335 to 156,152 9757 mRNA_1390: 9758 - KOG0988 RNA-directed RNA polymerase QDE-1 required for posttranscriptional gene silencing and RNA interference, at 156,559 to 159,898 9758 mRNA_1391: 9759 - HMMPfam-Phosphotransferase enzyme family-PF01636,SUPERFAMILY--SSF56112, at 160,091 to 161,111 9759 mRNA_1392: 9760 - HMMPfam-DASH complex subunit Dam1-PF08653, at 161,776 to 163,110 9760
Group Condition mRNA_1388 mRNA_1389 mRNA_1390 mRNA_1391 mRNA_1392
9756 9757 9758 9759 9760
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.2 -0.2 -0.2 -1.8 +0.3
facs on lipid accumulation Sorted High BODIPY Gate -0.1 +0.2 -0.3 -0.4 -0.8
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 -0.1 +0.1 -1.0 +0.0
auxotrophy YNB Glucose 75mM Methionine +0.0 +0.1 -0.0 -0.8 -0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.1 -0.1 -0.0 -0.5 -0.2
growth on ricinoleic acid YNB Glucose -0.1 +0.0 +0.1 -0.2 -0.5
facs on lipid accumulation Sorted Low BODIPY Gate +0.3 +0.2 -0.3 -0.3 -0.4
facs on lipid accumulation Sorted No BODIPY Gate +0.1 +0.1 -0.1 +0.1 -0.7
ypd buoyancy 40 hrs Low Buoyancy -0.1 -0.2 +0.2 -0.0 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 -0.0 +0.0 -0.3 +0.1
growth on oleic acid YNB Glucose -0.1 +0.1 -0.3 +0.1 +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 +0.1 +0.3 -0.2 -0.3
ypd buoyancy 40 hrs High Buoyancy -0.1 +0.0 +0.1 +0.2 -0.3
auxotrophy YNB Glucose -0.0 +0.1 +0.3 -0.1 -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.0 -0.1 +0.2 +0.2 -0.0
auxotrophy YNB Glucose 75mM Arginine -0.0 +0.1 -0.1 -0.1 +0.4
auxotrophy Dropout Complete +0.0 +0.0 -0.0 +0.2 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 +0.3 +0.1 +0.2 +0.0
growth on oleic acid YNB Oleic Acid -0.0 +0.2 -0.1 +0.1 +0.3
growth on ypd YPD -0.3 +0.2 +0.1 +0.5 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.1 -0.1 +0.2 -0.4 +1.3
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