Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9757 and 9758 are separated by 406 nucleotides9758 and 9759 are separated by 192 nucleotides9759 and 9760 are separated by 664 nucleotides9760 and 9761 are separated by 152 nucleotides mRNA_1389: 9757 - Hypothetical Protein, at 155,335 to 156,152 9757 mRNA_1390: 9758 - KOG0988 RNA-directed RNA polymerase QDE-1 required for posttranscriptional gene silencing and RNA interference, at 156,559 to 159,898 9758 mRNA_1391: 9759 - HMMPfam-Phosphotransferase enzyme family-PF01636,SUPERFAMILY--SSF56112, at 160,091 to 161,111 9759 mRNA_1392: 9760 - HMMPfam-DASH complex subunit Dam1-PF08653, at 161,776 to 163,110 9760 mRNA_1393: 9761 - Hypothetical Protein, at 163,263 to 164,488 9761
Group Condition mRNA_1389 mRNA_1390 mRNA_1391 mRNA_1392 mRNA_1393
9757 9758 9759 9760 9761
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 -0.3 -0.3 -0.4 -0.7
facs on lipid accumulation Sorted High BODIPY Gate +0.2 -0.3 -0.4 -0.8 +0.1
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.0 -0.8 -0.0 -0.2
ypd buoyancy 40 hrs Low Buoyancy -0.2 +0.2 -0.0 -0.2 -0.5
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 +0.1 +0.2 +0.0 -1.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 -0.2 -1.8 +0.3 +1.4
facs on lipid accumulation Sorted No BODIPY Gate +0.1 -0.1 +0.1 -0.7 +0.0
growth on oleic acid YNB Glucose +0.1 -0.3 +0.1 +0.1 -0.5
growth on oleic acid YNB Oleic Acid +0.2 -0.1 +0.1 +0.3 -0.9
auxotrophy YNB Glucose +0.1 +0.3 -0.1 -0.2 +0.0
auxotrophy YNB Glucose 75mM Arginine +0.1 -0.1 -0.1 +0.4 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid -0.0 +0.0 -0.3 +0.1 +0.3
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.2 +0.2 -0.0 +0.1
ypd buoyancy 40 hrs High Buoyancy +0.0 +0.1 +0.2 -0.3 +0.4
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.1 -0.0 -0.5 -0.2 +1.4
growth on ricinoleic acid YNB Glucose +0.0 +0.1 -0.2 -0.5 +1.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 +0.1 -1.0 +0.0 +1.7
growth on ypd YPD +0.2 +0.1 +0.5 +0.1 -0.1
auxotrophy Dropout Complete +0.0 -0.0 +0.2 +0.1 +0.6
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 +0.2 -0.4 +1.3 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 +0.3 -0.2 -0.3 +1.6
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