Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9758 and 9759 are separated by 192 nucleotides9759 and 9760 are separated by 664 nucleotides9760 and 9761 are separated by 152 nucleotides9761 and 9762 are separated by 12 nucleotides mRNA_1390: 9758 - KOG0988 RNA-directed RNA polymerase QDE-1 required for posttranscriptional gene silencing and RNA interference, at 156,559 to 159,898 9758 mRNA_1391: 9759 - HMMPfam-Phosphotransferase enzyme family-PF01636,SUPERFAMILY--SSF56112, at 160,091 to 161,111 9759 mRNA_1392: 9760 - HMMPfam-DASH complex subunit Dam1-PF08653, at 161,776 to 163,110 9760 mRNA_1393: 9761 - Hypothetical Protein, at 163,263 to 164,488 9761 mRNA_1394: 9762 - K04907 KCNH4 potassium voltage-gated channel Eag-related subfamily H member 4, at 164,501 to 165,028 9762
Group Condition mRNA_1390 mRNA_1391 mRNA_1392 mRNA_1393 mRNA_1394
9758 9759 9760 9761 9762
facs on lipid accumulation Sorted High BODIPY Gate -0.3 -0.4 -0.8 +0.1 -0.6
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 -0.3 -0.4 -0.7 +0.4
growth on oleic acid YNB Glucose -0.3 +0.1 +0.1 -0.5 +0.2
growth on oleic acid YNB Oleic Acid -0.1 +0.1 +0.3 -0.9 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 +0.2 +0.0 -1.2 +0.7
auxotrophy YNB Glucose 75mM Methionine -0.0 -0.8 -0.0 -0.2 +0.8
facs on lipid accumulation Sorted No BODIPY Gate -0.1 +0.1 -0.7 +0.0 +0.6
ypd buoyancy 40 hrs High Buoyancy +0.1 +0.2 -0.3 +0.4 -0.5
auxotrophy YNB Glucose 75mM Arginine -0.1 -0.1 +0.4 -0.2 -0.1
ypd buoyancy 40 hrs Low Buoyancy +0.2 -0.0 -0.2 -0.5 +0.6
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 -1.8 +0.3 +1.4 +0.4
auxotrophy YNB Glucose +0.3 -0.1 -0.2 +0.0 +0.6
growth on ricinoleic acid YNB Methylricinoleic Acid +0.0 -0.3 +0.1 +0.3 +0.5
ypd buoyancy 40 hrs Median Buoyancy +0.2 +0.2 -0.0 +0.1 +0.5
growth on ricinoleic acid YNB Glucose +0.1 -0.2 -0.5 +1.2 +0.5
growth on ypd YPD +0.1 +0.5 +0.1 -0.1 +0.6
auxotrophy Dropout Complete -0.0 +0.2 +0.1 +0.6 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 -1.0 +0.0 +1.7 +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.0 -0.5 -0.2 +1.4 +0.8
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.3 -0.2 -0.3 +1.6 +0.6
ypd buoyancy 40 hrs No Buoyancy Separation +0.2 -0.4 +1.3 +0.2 +0.8
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