Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9833 and 9834 are separated by 90 nucleotides9834 and 9835 are separated by 359 nucleotides9835 and 9836 are separated by 1727 nucleotides9836 and 9837 are separated by 1489 nucleotides mRNA_1465: 9833 - Hypothetical Protein, at 352,394 to 352,564 9833 mRNA_1466: 9834 - BLAST putative catalytic domain thiamin pyrophosphokinase [..., at 352,655 to 354,087 9834 mRNA_1467: 9835 - HMMPfam-Mannosyl oligosaccharide glucosidase-PF03200,SUPERFAMILY--SSF48208, at 354,447 to 358,959 9835 mRNA_1468: 9836 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 360,687 to 364,959 9836 mRNA_1469: 9837 - KOG1200 Mitochondrial/plastidial beta-ketoacyl-ACP reductase, at 366,449 to 367,493 9837
Group Condition mRNA_1465 mRNA_1466 mRNA_1467 mRNA_1468 mRNA_1469
9833 9834 9835 9836 9837
growth on ricinoleic acid YNB Ricinoleic Acid N.D. -0.1 -3.3 -2.9 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +0.1 -1.0 -3.4 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -1.6 -0.9 -1.2 N.D.
growth on oleic acid YNB Oleic Acid N.D. -0.9 -0.5 -1.4 N.D.
auxotrophy Dropout Complete N.D. -0.5 -0.6 -1.2 N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. +0.2 -0.5 -0.9 N.D.
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.2 -0.6 -0.4 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. -0.4 -0.3 -0.4 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. -0.2 -0.1 -0.7 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. +0.3 -0.1 -0.7 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.1 -0.1 -0.5 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.4 -0.4 -0.4 N.D.
auxotrophy YNB Glucose N.D. +0.0 -0.2 -0.2 N.D.
growth on ricinoleic acid YNB Glucose N.D. +0.0 +0.0 -0.3 N.D.
growth on oleic acid YNB Glucose N.D. -0.2 +0.0 +0.4 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.1 +0.1 +0.1 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. +0.7 -0.4 -0.0 N.D.
growth on ypd YPD N.D. +0.2 -0.2 +0.4 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. +0.1 +0.3 +0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. +0.3 +0.5 -0.3 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.8 +0.2 -0.0 N.D.
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