Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9836 and 9837 are separated by 1489 nucleotides9837 and 9838 are separated by 707 nucleotides9838 and 9839 are separated by 253 nucleotides9839 and 9840 are separated by 475 nucleotides mRNA_1468: 9836 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 360,687 to 364,959 9836 mRNA_1469: 9837 - KOG1200 Mitochondrial/plastidial beta-ketoacyl-ACP reductase, at 366,449 to 367,493 9837 mRNA_1470: 9838 - K18592 GGT1_5, CD224 gamma-glutamyltranspeptidase / glutathione hydrolase / leukotriene-C4 hydrolase, at 368,201 to 370,453 9838 mRNA_1471: 9839 - K08874 TRRAP transformation/transcription domain-associated protein, at 370,707 to 382,935 9839 mRNA_1472: 9840 - HMMPfam-Zinc finger, C3HC4 type (RING finger)-PF00097,ProSitePatterns-Zinc finger RING-type signature.-PS00518,SMART-Ring finger-SM00184,SUPERFAMILY--SSF57850, at 383,411 to 384,622 9840
Group Condition mRNA_1468 mRNA_1469 mRNA_1470 mRNA_1471 mRNA_1472
9836 9837 9838 9839 9840
growth on ricinoleic acid YNB Methylricinoleic Acid -3.4 N.D. -0.3 -1.2 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid -2.9 N.D. -0.4 -1.3 -0.0
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 N.D. -0.5 -2.6 -0.6
growth on oleic acid YNB Oleic Acid -1.4 N.D. +0.2 -2.4 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation -1.2 N.D. +0.9 -3.1 -0.0
auxotrophy Dropout Complete -1.2 N.D. +0.1 -2.2 +0.1
growth on oleic acid YNB Glucose +0.4 N.D. +0.0 -3.0 -0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 N.D. +0.3 -1.7 -0.4
facs on lipid accumulation Sorted High BODIPY Gate -0.9 N.D. -0.7 -0.3 +0.2
auxotrophy YNB Glucose 75mM Arginine -0.7 N.D. +0.4 -1.4 +0.1
growth on ricinoleic acid YNB Glucose -0.3 N.D. +0.0 -1.5 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 N.D. +0.1 -1.0 -0.6
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.7 N.D. +0.3 -0.9 -0.1
facs on lipid accumulation Sorted No BODIPY Gate -0.4 N.D. -0.2 +0.1 -0.4
ypd buoyancy 40 hrs Median Buoyancy -0.5 N.D. +0.2 -0.5 +0.2
ypd buoyancy 40 hrs High Buoyancy +0.1 N.D. +0.3 -0.5 +0.0
auxotrophy YNB Glucose -0.2 N.D. +0.1 +0.1 -0.0
ypd buoyancy 40 hrs Low Buoyancy -0.3 N.D. +0.1 +0.0 +0.1
auxotrophy YNB Glucose 75mM Methionine -0.4 N.D. -0.0 +0.5 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.0 N.D. +0.5 +0.0 -0.1
growth on ypd YPD +0.4 N.D. -0.5 +0.8 +0.4
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