Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9853 and 9854 are separated by 84 nucleotides9854 and 9855 are separated by 641 nucleotides9855 and 9856 are separated by 258 nucleotides9856 and 9857 are separated by 572 nucleotides mRNA_1485: 9853 - HMMPfam-WW domain-PF00397,ProSitePatterns-WW/rsp5/WWP domain signature.-PS01159,ProSiteProfiles-WW/rsp5/WWP domain profile.-PS50020,SMART-Domain with 2 conserved Trp (W) residues-SM00456,SUPERFAMILY--SSF51045, at 412,872 to 413,936 9853 mRNA_1486: 9854 - K11085 msbA ATP-binding cassette, subfamily B, bacterial MsbA, at 414,021 to 419,409 9854 mRNA_1487: 9855 - K01883 CARS, cysS cysteinyl-tRNA synthetase, at 420,051 to 423,071 9855 mRNA_1488: 9856 - K15371 GDH2 glutamate dehydrogenase, at 423,330 to 427,670 9856 mRNA_1489: 9857 - K09022 ridA, tdcF 2-iminobutanoate/2-iminopropanoate deaminase, at 428,243 to 429,095 9857
Group Condition mRNA_1485 mRNA_1486 mRNA_1487 mRNA_1488 mRNA_1489
9853 9854 9855 9856 9857
ypd buoyancy 40 hrs No Buoyancy Separation -1.3 -0.1 -1.7 -0.1 -0.5
facs on lipid accumulation Sorted High BODIPY Gate -0.1 -0.2 -0.6 -0.4 -0.9
facs on lipid accumulation Sorted No BODIPY Gate -0.7 -0.1 +0.4 -0.6 -0.8
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 +0.0 -0.7 +0.1 -0.7
facs on lipid accumulation Sorted Low BODIPY Gate -0.3 -0.3 +0.1 -0.3 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.5 -0.1 -0.0 +0.1 -0.3
ypd buoyancy 40 hrs Median Buoyancy -0.1 +0.1 -0.1 -0.1 -0.3
ypd buoyancy 40 hrs Low Buoyancy +0.1 -0.1 +0.3 -0.0 -0.5
growth on ypd YPD -0.1 +0.1 -0.2 -0.2 +0.1
growth on oleic acid YNB Oleic Acid +0.3 -0.0 +0.1 -0.6 +0.0
auxotrophy Dropout Complete -0.1 -0.2 +0.6 -0.2 -0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.8 +0.1 +0.3 -0.1 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 -0.0 +0.3 +0.0 -0.3
auxotrophy YNB Glucose 75mM Methionine -0.2 -0.1 +0.5 +0.1 -0.3
growth on oleic acid YNB Glucose +0.1 +0.1 +0.1 -0.2 -0.3
ypd buoyancy 40 hrs High Buoyancy -0.2 +0.1 +0.4 +0.0 -0.3
growth on ricinoleic acid YNB Glucose +0.5 -0.0 -0.1 -0.1 +0.1
auxotrophy YNB Glucose 75mM Arginine +0.2 -0.1 +0.8 +0.0 -0.5
auxotrophy YNB Glucose +0.1 -0.2 +0.6 +0.1 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.5 -0.0 +0.9 -0.5 -0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.7 -0.1 +0.6 -0.2 -0.2
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