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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
9853 and 9854 are separated by 84 nucleotides
9854 and 9855 are separated by 641 nucleotides
9855 and 9856 are separated by 258 nucleotides
9856 and 9857 are separated by 572 nucleotides
mRNA_1485: 9853 - HMMPfam-WW domain-PF00397,ProSitePatterns-WW/rsp5/WWP domain signature.-PS01159,ProSiteProfiles-WW/rsp5/WWP domain profile.-PS50020,SMART-Domain with 2 conserved Trp (W) residues-SM00456,SUPERFAMILY--SSF51045, at 412,872 to 413,936
9853
mRNA_1486: 9854 - K11085 msbA ATP-binding cassette, subfamily B, bacterial MsbA, at 414,021 to 419,409
9854
mRNA_1487: 9855 - K01883 CARS, cysS cysteinyl-tRNA synthetase, at 420,051 to 423,071
9855
mRNA_1488: 9856 - K15371 GDH2 glutamate dehydrogenase, at 423,330 to 427,670
9856
mRNA_1489: 9857 - K09022 ridA, tdcF 2-iminobutanoate/2-iminopropanoate deaminase, at 428,243 to 429,095
9857
Group
Condition
mRNA
_1485
mRNA
_1486
mRNA
_1487
mRNA
_1488
mRNA
_1489
9853
9854
9855
9856
9857
ypd buoyancy
40 hrs No Buoyancy Separation
-1.3
-0.1
-1.7
-0.1
-0.5
facs on lipid accumulation
Sorted High BODIPY Gate
-0.1
-0.2
-0.6
-0.4
-0.9
facs on lipid accumulation
Sorted No BODIPY Gate
-0.7
-0.1
+0.4
-0.6
-0.8
buoyancy on lipid accumulation
40 hrs High Buoyancy
-0.2
+0.0
-0.7
+0.1
-0.7
facs on lipid accumulation
Sorted Low BODIPY Gate
-0.3
-0.3
+0.1
-0.3
-0.1
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.5
-0.1
-0.0
+0.1
-0.3
ypd buoyancy
40 hrs Median Buoyancy
-0.1
+0.1
-0.1
-0.1
-0.3
ypd buoyancy
40 hrs Low Buoyancy
+0.1
-0.1
+0.3
-0.0
-0.5
growth on ypd
YPD
-0.1
+0.1
-0.2
-0.2
+0.1
growth on oleic acid
YNB Oleic Acid
+0.3
-0.0
+0.1
-0.6
+0.0
auxotrophy
Dropout Complete
-0.1
-0.2
+0.6
-0.2
-0.3
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.8
+0.1
+0.3
-0.1
+0.3
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.1
-0.0
+0.3
+0.0
-0.3
auxotrophy
YNB Glucose 75mM Methionine
-0.2
-0.1
+0.5
+0.1
-0.3
growth on oleic acid
YNB Glucose
+0.1
+0.1
+0.1
-0.2
-0.3
ypd buoyancy
40 hrs High Buoyancy
-0.2
+0.1
+0.4
+0.0
-0.3
growth on ricinoleic acid
YNB Glucose
+0.5
-0.0
-0.1
-0.1
+0.1
auxotrophy
YNB Glucose 75mM Arginine
+0.2
-0.1
+0.8
+0.0
-0.5
auxotrophy
YNB Glucose
+0.1
-0.2
+0.6
+0.1
-0.1
growth on ricinoleic acid
YNB Methylricinoleic Acid
+0.5
-0.0
+0.9
-0.5
-0.2
growth on ricinoleic acid
YNB Ricinoleic Acid
+0.7
-0.1
+0.6
-0.2
-0.2
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