Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9863 and 9864 are separated by 525 nucleotides9864 and 9865 are separated by 233 nucleotides9865 and 9866 are separated by 617 nucleotides9866 and 9867 are separated by 468 nucleotides mRNA_1495: 9863 - Hypothetical Protein, at 444,439 to 447,391 9863 mRNA_1496: 9864 - HMMPfam-Major Facilitator Superfamily-PF07690,PRINTS-Tetracycline resistance protein TetB signature-PR01036,ProSiteProfiles-Major facilitator superfamily (MFS) profile.-PS50850,SUPERFAMILY--SSF103473, at 447,917 to 450,188 9864 mRNA_1497: 9865 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 450,422 to 453,504 9865 mRNA_1498: 9866 - K11129 NHP2, NOLA2 H/ACA ribonucleoprotein complex subunit 2, at 454,122 to 455,195 9866 mRNA_1499: 9867 - KOG1231 Proteins containing the FAD binding domain, at 455,664 to 457,570 9867
Group Condition mRNA_1495 mRNA_1496 mRNA_1497 mRNA_1498 mRNA_1499
9863 9864 9865 9866 9867
growth on ricinoleic acid YNB Methylricinoleic Acid -0.5 -0.1 -0.3 N.D. -0.3
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 -0.9 +0.6 N.D. -0.4
auxotrophy YNB Glucose 75mM Methionine -0.3 +0.1 -0.2 N.D. -0.2
growth on ricinoleic acid YNB Glucose +0.1 -0.4 -0.1 N.D. -0.2
auxotrophy Dropout Complete -0.3 +0.2 +0.0 N.D. -0.2
auxotrophy YNB Glucose -0.2 +0.2 -0.1 N.D. -0.2
ypd buoyancy 40 hrs Median Buoyancy -0.5 +0.2 +0.0 N.D. +0.1
growth on oleic acid YNB Oleic Acid -0.3 +0.1 +0.2 N.D. -0.1
growth on oleic acid YNB Glucose -0.3 +0.2 +0.0 N.D. +0.0
ypd buoyancy 40 hrs Low Buoyancy -0.0 +0.0 -0.1 N.D. -0.0
facs on lipid accumulation Sorted No BODIPY Gate -0.3 +0.2 +0.1 N.D. -0.0
auxotrophy YNB Glucose 75mM Arginine +0.1 +0.2 -0.2 N.D. -0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.3 -0.3 +0.1 N.D. +0.1
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 +0.1 +0.4 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 -0.0 +0.0 N.D. +0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.0 +0.3 +0.4 N.D. +0.0
ypd buoyancy 40 hrs High Buoyancy -0.2 +0.5 +0.2 N.D. +0.2
growth on ypd YPD +0.1 +0.3 +0.3 N.D. +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.5 -0.3 +0.6 N.D. +0.1
facs on lipid accumulation Sorted Low BODIPY Gate +0.6 +0.6 -0.1 N.D. +0.3
facs on lipid accumulation Sorted High BODIPY Gate +0.3 +0.6 +0.5 N.D. +0.3
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