Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9865 and 9866 are separated by 617 nucleotides9866 and 9867 are separated by 468 nucleotides9867 and 9868 are separated by 699 nucleotides9868 and 9869 are separated by 231 nucleotides mRNA_1497: 9865 - HMMPfam-Fungal Zn(2)-Cys(6) binuclear cluster domain-PF00172,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SUPERFAMILY--SSF57701, at 450,422 to 453,504 9865 mRNA_1498: 9866 - K11129 NHP2, NOLA2 H/ACA ribonucleoprotein complex subunit 2, at 454,122 to 455,195 9866 mRNA_1499: 9867 - KOG1231 Proteins containing the FAD binding domain, at 455,664 to 457,570 9867 mRNA_1500: 9868 - K05770 TSPO, BZRP benzodiazapine receptor, at 458,270 to 459,039 9868 mRNA_1501: 9869 - Hypothetical Protein, at 459,271 to 459,744 9869
Group Condition mRNA_1497 mRNA_1498 mRNA_1499 mRNA_1500 mRNA_1501
9865 9866 9867 9868 9869
facs on lipid accumulation Sorted Low BODIPY Gate -0.1 N.D. +0.3 -2.5 -0.2
growth on oleic acid YNB Oleic Acid +0.2 N.D. -0.1 -0.6 -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.1 N.D. -0.0 -0.2 -0.4
growth on ricinoleic acid YNB Methylricinoleic Acid -0.3 N.D. -0.3 +0.5 -0.2
auxotrophy YNB Glucose 75mM Methionine -0.2 N.D. -0.2 +0.4 -0.1
growth on ricinoleic acid YNB Glucose -0.1 N.D. -0.2 +0.3 +0.0
ypd buoyancy 40 hrs Low Buoyancy -0.1 N.D. -0.0 +0.1 +0.1
growth on oleic acid YNB Glucose +0.0 N.D. +0.0 +0.2 -0.1
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. +0.2 -0.4 +0.2
auxotrophy YNB Glucose -0.1 N.D. -0.2 +0.4 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 N.D. +0.1 +0.2 -0.2
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.4 N.D. +0.0 +0.0 -0.1
auxotrophy Dropout Complete +0.0 N.D. -0.2 +0.5 -0.0
facs on lipid accumulation Sorted High BODIPY Gate +0.5 N.D. +0.3 -0.3 -0.1
auxotrophy YNB Glucose 75mM Arginine -0.2 N.D. -0.1 +0.6 +0.1
ypd buoyancy 40 hrs Median Buoyancy +0.0 N.D. +0.1 +0.2 +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +0.6 N.D. -0.4 +0.3 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.4 N.D. -0.2 +0.6 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.0 N.D. +0.1 +1.0 -0.1
growth on ypd YPD +0.3 N.D. +0.0 +1.0 -0.1
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.6 N.D. +0.1 +0.7 -0.1
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