Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9887 and 9888 are separated by 316 nucleotides9888 and 9889 are separated by 269 nucleotides9889 and 9890 are separated by 662 nucleotides9890 and 9891 are separated by 146 nucleotides mRNA_1519: 9887 - K03434 PIGL N-acetylglucosaminylphosphatidylinositol deacetylase, at 498,486 to 499,593 9887 mRNA_1520: 9888 - K00765 hisG ATP phosphoribosyltransferase, at 499,910 to 501,221 9888 mRNA_1521: 9889 - HMMPfam-DNA polymerase delta, subunit 4-PF04081, at 501,491 to 502,163 9889 mRNA_1522: 9890 - K06685 MOB1, Mats MOB kinase activator 1, at 502,826 to 504,110 9890 mRNA_1523: 9891 - Hypothetical Protein, at 504,257 to 504,927 9891
Group Condition mRNA_1519 mRNA_1520 mRNA_1521 mRNA_1522 mRNA_1523
9887 9888 9889 9890 9891
ypd buoyancy 40 hrs No Buoyancy Separation -0.2 N.D. N.D. -2.0 -0.4
growth on ypd YPD +0.0 N.D. N.D. -0.3 -0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.2 N.D. N.D. -0.3 -0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 N.D. N.D. +0.5 -0.4
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. N.D. +0.5 -0.3
auxotrophy Dropout Complete -0.0 N.D. N.D. -0.0 +0.2
ypd buoyancy 40 hrs Low Buoyancy +0.5 N.D. N.D. -0.1 -0.2
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. N.D. -0.1 +0.1
auxotrophy YNB Glucose 75mM Methionine +0.1 N.D. N.D. +0.1 +0.0
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 N.D. N.D. +0.1 +0.2
facs on lipid accumulation Sorted No BODIPY Gate +0.2 N.D. N.D. +0.4 -0.3
auxotrophy YNB Glucose 75mM Arginine +0.4 N.D. N.D. +0.0 +0.1
auxotrophy YNB Glucose +0.0 N.D. N.D. +0.3 +0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.5 N.D. N.D. +0.3 -0.4
growth on oleic acid YNB Glucose +0.4 N.D. N.D. +0.1 -0.1
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. N.D. +0.4 +0.0
facs on lipid accumulation Sorted High BODIPY Gate +0.9 N.D. N.D. +0.1 -0.1
growth on ricinoleic acid YNB Methylricinoleic Acid +0.7 N.D. N.D. +0.2 -0.0
growth on ricinoleic acid YNB Glucose +0.4 N.D. N.D. +0.5 +0.3
growth on oleic acid YNB Oleic Acid +0.8 N.D. N.D. +0.3 +0.0
growth on ricinoleic acid YNB Ricinoleic Acid +0.9 N.D. N.D. +0.4 -0.1
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