Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9892 and 9893 are separated by 133 nucleotides9893 and 9894 are separated by 387 nucleotides9894 and 9895 are separated by 1141 nucleotides9895 and 9896 are separated by 534 nucleotides mRNA_1524: 9892 - K07178 RIOK1 RIO kinase 1, at 505,162 to 507,225 9892 mRNA_1525: 9893 - K08267 RICTOR rapamycin-insensitive companion of mTOR, at 507,359 to 512,878 9893 mRNA_1526: 9894 - K02880 RP-L17e, RPL17 large subunit ribosomal protein L17e, at 513,266 to 514,362 9894 mRNA_1527: 9895 - KOG3272 Predicted coiled-coil protein, at 515,504 to 516,509 9895 mRNA_1528: 9896 - K06030 MFN mitofusin, at 517,044 to 520,163 9896
Group Condition mRNA_1524 mRNA_1525 mRNA_1526 mRNA_1527 mRNA_1528
9892 9893 9894 9895 9896
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. N.D. -1.0 N.D.
growth on ypd YPD N.D. N.D. N.D. -0.9 N.D.
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. N.D. -0.8 N.D.
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. N.D. -0.5 N.D.
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. N.D. -0.3 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. N.D. -0.3 N.D.
auxotrophy Dropout Complete N.D. N.D. N.D. -0.3 N.D.
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. N.D. -0.1 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. N.D. -0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. N.D. -0.1 N.D.
auxotrophy YNB Glucose N.D. N.D. N.D. +0.0 N.D.
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. N.D. +0.1 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. N.D. +0.2 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. N.D. +0.3 N.D.
growth on ricinoleic acid YNB Glucose N.D. N.D. N.D. +0.3 N.D.
growth on oleic acid YNB Glucose N.D. N.D. N.D. +0.4 N.D.
growth on oleic acid YNB Oleic Acid N.D. N.D. N.D. +0.4 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. N.D. +0.5 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. N.D. +0.7 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. N.D. +0.7 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. N.D. +0.8 N.D.
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