Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9897 and 9898 are separated by 1991 nucleotides9898 and 9899 are separated by 288 nucleotides9899 and 9900 are separated by 83 nucleotides9900 and 9901 are separated by 200 nucleotides mRNA_1529: 9897 - K12605 CNOT2, NOT2 CCR4-NOT transcription complex subunit 2, at 520,532 to 522,546 9897 mRNA_1530: 9898 - K15296 NAPA, SNAPA, SEC17 alpha-soluble NSF attachment protein, at 524,538 to 525,796 9898 mRNA_1531: 9899 - KOG1852 Cell cycle-associated protein, at 526,085 to 527,071 9899 mRNA_1532: 9900 - K00766 trpD anthranilate phosphoribosyltransferase, at 527,155 to 528,581 9900 mRNA_1533: 9901 - K04649 HIP2, UBC1 ubiquitin-conjugating enzyme (huntingtin interacting protein 2), at 528,782 to 530,057 9901
Group Condition mRNA_1529 mRNA_1530 mRNA_1531 mRNA_1532 mRNA_1533
9897 9898 9899 9900 9901
growth on ricinoleic acid YNB Ricinoleic Acid -1.1 N.D. -0.1 N.D. -1.0
auxotrophy YNB Glucose 75mM Arginine -0.7 N.D. -0.3 N.D. -0.9
growth on oleic acid YNB Glucose -1.1 N.D. -0.1 N.D. -0.6
growth on ricinoleic acid YNB Glucose -1.1 N.D. +0.1 N.D. -0.4
growth on oleic acid YNB Oleic Acid -0.3 N.D. -0.0 N.D. -0.9
auxotrophy YNB Glucose 75mM Methionine -0.5 N.D. +0.0 N.D. -0.6
growth on ricinoleic acid YNB Methylricinoleic Acid -1.0 N.D. +0.3 N.D. -0.5
facs on lipid accumulation Sorted No BODIPY Gate -1.6 N.D. +0.0 N.D. +0.5
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.2 N.D. -0.6 N.D. -0.3
auxotrophy YNB Glucose -0.3 N.D. -0.1 N.D. -0.6
facs on lipid accumulation Sorted Low BODIPY Gate -1.3 N.D. +0.2 N.D. +0.3
growth on ypd YPD -0.4 N.D. -0.1 N.D. -0.2
ypd buoyancy 40 hrs High Buoyancy -0.3 N.D. +0.2 N.D. -0.6
facs on lipid accumulation Sorted High BODIPY Gate +0.2 N.D. +0.2 N.D. -0.9
ypd buoyancy 40 hrs Median Buoyancy +0.1 N.D. +0.4 N.D. -0.7
auxotrophy Dropout Complete +0.4 N.D. -0.2 N.D. -0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.4 N.D. -0.2 N.D. -0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 N.D. -0.3 N.D. +0.4
ypd buoyancy 40 hrs Low Buoyancy -0.5 N.D. -0.0 N.D. +0.7
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.5 N.D. +0.1 N.D. +0.1
ypd buoyancy 40 hrs No Buoyancy Separation +1.3 N.D. +0.3 N.D. -0.9
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