Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9899 and 9900 are separated by 83 nucleotides9900 and 9901 are separated by 200 nucleotides9901 and 9902 are separated by 214 nucleotides9902 and 9903 are separated by 459 nucleotides mRNA_1531: 9899 - KOG1852 Cell cycle-associated protein, at 526,085 to 527,071 9899 mRNA_1532: 9900 - K00766 trpD anthranilate phosphoribosyltransferase, at 527,155 to 528,581 9900 mRNA_1533: 9901 - K04649 HIP2, UBC1 ubiquitin-conjugating enzyme (huntingtin interacting protein 2), at 528,782 to 530,057 9901 mRNA_1534: 9902 - K12493 ARFGAP2_3 ADP-ribosylation factor GTPase-activating protein 2/3, at 530,272 to 532,362 9902 mRNA_1535: 9903 - K12471 EPN epsin, at 532,822 to 534,645 9903
Group Condition mRNA_1531 mRNA_1532 mRNA_1533 mRNA_1534 mRNA_1535
9899 9900 9901 9902 9903
facs on lipid accumulation Sorted High BODIPY Gate +0.2 N.D. -0.9 -0.5 -0.6
auxotrophy YNB Glucose 75mM Arginine -0.3 N.D. -0.9 +0.5 -0.2
ypd buoyancy 40 hrs High Buoyancy +0.2 N.D. -0.6 +0.1 -0.5
facs on lipid accumulation Sorted No BODIPY Gate +0.0 N.D. +0.5 +0.0 -1.0
growth on oleic acid YNB Glucose -0.1 N.D. -0.6 +0.6 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.6 N.D. -0.3 +0.6 +0.0
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 N.D. -0.9 -0.0 +0.4
auxotrophy YNB Glucose -0.1 N.D. -0.6 +0.5 -0.0
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.1 N.D. +0.1 -0.2 -0.0
auxotrophy YNB Glucose 75mM Methionine +0.0 N.D. -0.6 +0.3 +0.3
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.3 N.D. +0.4 -0.3 +0.2
growth on ypd YPD -0.1 N.D. -0.2 -0.3 +0.6
ypd buoyancy 40 hrs Median Buoyancy +0.4 N.D. -0.7 -0.2 +0.5
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 N.D. -0.2 +0.4 +0.1
growth on oleic acid YNB Oleic Acid -0.0 N.D. -0.9 +1.7 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate +0.2 N.D. +0.3 +0.5 -0.4
auxotrophy Dropout Complete -0.2 N.D. -0.3 +0.8 +0.2
growth on ricinoleic acid YNB Glucose +0.1 N.D. -0.4 +0.7 +0.3
growth on ricinoleic acid YNB Ricinoleic Acid -0.1 N.D. -1.0 +2.1 +0.5
ypd buoyancy 40 hrs Low Buoyancy -0.0 N.D. +0.7 +0.6 +0.4
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 N.D. -0.5 +1.7 +0.6
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