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Fitness for 5 genes in
Rhodosporidium toruloides IFO0880
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Top 21 experiments (either direction), sorted by average fitness
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500 nt
9899 and 9900 are separated by 83 nucleotides
9900 and 9901 are separated by 200 nucleotides
9901 and 9902 are separated by 214 nucleotides
9902 and 9903 are separated by 459 nucleotides
mRNA_1531: 9899 - KOG1852 Cell cycle-associated protein, at 526,085 to 527,071
9899
mRNA_1532: 9900 - K00766 trpD anthranilate phosphoribosyltransferase, at 527,155 to 528,581
9900
mRNA_1533: 9901 - K04649 HIP2, UBC1 ubiquitin-conjugating enzyme (huntingtin interacting protein 2), at 528,782 to 530,057
9901
mRNA_1534: 9902 - K12493 ARFGAP2_3 ADP-ribosylation factor GTPase-activating protein 2/3, at 530,272 to 532,362
9902
mRNA_1535: 9903 - K12471 EPN epsin, at 532,822 to 534,645
9903
Group
Condition
mRNA
_1531
mRNA
_1532
mRNA
_1533
mRNA
_1534
mRNA
_1535
9899
9900
9901
9902
9903
facs on lipid accumulation
Sorted High BODIPY Gate
+0.2
N.D.
-0.9
-0.5
-0.6
auxotrophy
YNB Glucose 75mM Arginine
-0.3
N.D.
-0.9
+0.5
-0.2
ypd buoyancy
40 hrs High Buoyancy
+0.2
N.D.
-0.6
+0.1
-0.5
facs on lipid accumulation
Sorted No BODIPY Gate
+0.0
N.D.
+0.5
+0.0
-1.0
growth on oleic acid
YNB Glucose
-0.1
N.D.
-0.6
+0.6
-0.3
buoyancy on lipid accumulation
40 hrs No Buoyancy Separation
-0.6
N.D.
-0.3
+0.6
+0.0
ypd buoyancy
40 hrs No Buoyancy Separation
+0.3
N.D.
-0.9
-0.0
+0.4
auxotrophy
YNB Glucose
-0.1
N.D.
-0.6
+0.5
-0.0
buoyancy on lipid accumulation
40 hrs High Buoyancy
+0.1
N.D.
+0.1
-0.2
-0.0
auxotrophy
YNB Glucose 75mM Methionine
+0.0
N.D.
-0.6
+0.3
+0.3
buoyancy on lipid accumulation
40 hrs Low Buoyancy
-0.3
N.D.
+0.4
-0.3
+0.2
growth on ypd
YPD
-0.1
N.D.
-0.2
-0.3
+0.6
ypd buoyancy
40 hrs Median Buoyancy
+0.4
N.D.
-0.7
-0.2
+0.5
buoyancy on lipid accumulation
40 hrs Median Buoyancy
-0.2
N.D.
-0.2
+0.4
+0.1
growth on oleic acid
YNB Oleic Acid
-0.0
N.D.
-0.9
+1.7
-0.3
facs on lipid accumulation
Sorted Low BODIPY Gate
+0.2
N.D.
+0.3
+0.5
-0.4
auxotrophy
Dropout Complete
-0.2
N.D.
-0.3
+0.8
+0.2
growth on ricinoleic acid
YNB Glucose
+0.1
N.D.
-0.4
+0.7
+0.3
growth on ricinoleic acid
YNB Ricinoleic Acid
-0.1
N.D.
-1.0
+2.1
+0.5
ypd buoyancy
40 hrs Low Buoyancy
-0.0
N.D.
+0.7
+0.6
+0.4
growth on ricinoleic acid
YNB Methylricinoleic Acid
+0.3
N.D.
-0.5
+1.7
+0.6
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