Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9910 and 9911 are separated by 514 nucleotides9911 and 9912 are separated by 1478 nucleotides9912 and 9913 are separated by 219 nucleotides9913 and 9914 are separated by 485 nucleotides mRNA_1542: 9910 - K01834 PGAM, gpmA 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase, at 551,474 to 552,455 9910 mRNA_1543: 9911 - Hypothetical Protein, at 552,970 to 553,858 9911 mRNA_1544: 9912 - K08745 SLC27A1_4, FATP1, FATP4 solute carrier family 27 (fatty acid transporter), member 1/4, at 555,337 to 558,196 9912 mRNA_1545: 9913 - Hypothetical Protein, at 558,416 to 558,832 9913 mRNA_1546: 9914 - K18040 PTPN23 tyrosine-protein phosphatase non-receptor type 23, at 559,318 to 562,481 9914
Group Condition mRNA_1542 mRNA_1543 mRNA_1544 mRNA_1545 mRNA_1546
9910 9911 9912 9913 9914
facs on lipid accumulation Sorted No BODIPY Gate N.D. -0.9 +0.2 -0.1 -1.9
facs on lipid accumulation Sorted Low BODIPY Gate N.D. -0.9 +0.0 +0.0 -1.9
facs on lipid accumulation Sorted High BODIPY Gate N.D. -0.2 -0.3 +0.1 -2.1
auxotrophy YNB Glucose N.D. +0.4 -0.3 -0.1 -1.1
auxotrophy YNB Glucose 75mM Methionine N.D. +0.2 -0.0 -0.1 -1.1
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. +0.2 -0.1 +0.1 -1.1
ypd buoyancy 40 hrs Low Buoyancy N.D. -1.1 -0.1 -0.2 +0.6
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. -0.4 +0.0 -0.1 -0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. +0.1 -0.1 -0.0 -0.5
auxotrophy YNB Glucose 75mM Arginine N.D. +0.5 +0.0 -0.1 -0.9
growth on ricinoleic acid YNB Ricinoleic Acid N.D. +0.4 -0.3 +0.1 -0.6
growth on oleic acid YNB Glucose N.D. +0.4 -0.0 +0.3 -1.0
growth on ricinoleic acid YNB Glucose N.D. +0.3 +0.1 +0.0 -0.8
auxotrophy Dropout Complete N.D. +0.3 -0.1 +0.0 -0.4
ypd buoyancy 40 hrs No Buoyancy Separation N.D. -0.6 -0.3 -0.6 +1.4
growth on oleic acid YNB Oleic Acid N.D. +1.2 -0.1 +0.2 -1.5
growth on ypd YPD N.D. +0.7 -0.0 -0.3 -0.0
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. +0.6 -0.5 +0.3 +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. +0.7 -0.1 -0.1 +0.3
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. +1.0 +0.3 -0.1 -0.3
ypd buoyancy 40 hrs High Buoyancy N.D. +0.3 +0.1 -0.1 +1.0
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