Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9939 and 9940 are separated by 505 nucleotides9940 and 9941 are separated by 534 nucleotides9941 and 9942 are separated by 161 nucleotides9942 and 9943 are separated by 566 nucleotides mRNA_1571: 9939 - K03695 clpB ATP-dependent Clp protease ATP-binding subunit ClpB, at 620,682 to 623,667 9939 mRNA_1572: 9940 - K02139 ATPeFF, ATP17 F-type H+-transporting ATPase subunit f, at 624,173 to 624,749 9940 mRNA_1573: 9941 - KOG2022 Nuclear transport receptor LGL2 (importin beta superfamily), at 625,284 to 628,967 9941 mRNA_1574: 9942 - K06867 K06867 uncharacterized protein, at 629,129 to 634,398 9942 mRNA_1575: 9943 - BLAST cipC protein [Aspergillus oryzae RIB40], at 634,965 to 635,420 9943
Group Condition mRNA_1571 mRNA_1572 mRNA_1573 mRNA_1574 mRNA_1575
9939 9940 9941 9942 9943
facs on lipid accumulation Sorted High BODIPY Gate -0.1 N.D. -0.1 -2.9 -0.9
facs on lipid accumulation Sorted No BODIPY Gate +0.2 N.D. +0.2 -2.3 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 N.D. -0.5 -1.1 -0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.3 N.D. -0.5 -1.8 +0.2
growth on ricinoleic acid YNB Ricinoleic Acid +0.5 N.D. -0.5 -2.1 +0.5
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.2 N.D. -0.4 -1.5 +0.2
growth on ricinoleic acid YNB Glucose +0.1 N.D. -0.1 -1.1 -0.0
auxotrophy YNB Glucose 75mM Arginine +0.5 N.D. -0.4 -0.4 -0.3
auxotrophy Dropout Complete +0.6 N.D. -0.1 -0.8 -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.3 N.D. -0.1 -0.4 +0.2
auxotrophy YNB Glucose +0.4 N.D. -0.2 -0.2 -0.4
ypd buoyancy 40 hrs No Buoyancy Separation -1.1 N.D. +0.7 +0.0 +0.0
ypd buoyancy 40 hrs Low Buoyancy -0.6 N.D. +0.1 +0.2 -0.0
auxotrophy YNB Glucose 75mM Methionine +0.4 N.D. -0.3 +0.0 -0.3
facs on lipid accumulation Sorted Low BODIPY Gate +0.5 N.D. +0.3 -1.1 +0.3
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 N.D. +0.2 -0.2 +0.2
growth on ypd YPD -0.8 N.D. -0.0 +1.1 -0.1
growth on oleic acid YNB Oleic Acid -0.1 N.D. -0.2 +0.4 +0.1
ypd buoyancy 40 hrs High Buoyancy -0.0 N.D. -0.2 +0.1 +0.5
growth on oleic acid YNB Glucose +0.1 N.D. -0.1 +0.4 +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.2 N.D. +0.2 +0.6 -0.1
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