Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9957 and 9958 are separated by 735 nucleotides9958 and 9959 are separated by 236 nucleotides9959 and 9960 are separated by 550 nucleotides9960 and 9961 are separated by 1031 nucleotides mRNA_1589: 9957 - Hypothetical Protein, at 666,700 to 668,966 9957 mRNA_1590: 9958 - KOG0586 Serine/threonine protein kinase, at 669,702 to 673,169 9958 mRNA_1591: 9959 - K01835 pgm phosphoglucomutase, at 673,406 to 675,711 9959 mRNA_1592: 9960 - HMMPfam-bZIP transcription factor-PF00170,ProSitePatterns-Basic-leucine zipper (bZIP) domain signature.-PS00036,ProSiteProfiles-Basic-leucine zipper (bZIP) domain profile.-PS50217,SMART-basic region leucin zipper-SM00338,SUPERFAMILY--SSF57959, at 676,262 to 677,615 9960 mRNA_1593: 9961 - KOG4366 Predicted thioesterase, at 678,647 to 679,781 9961
Group Condition mRNA_1589 mRNA_1590 mRNA_1591 mRNA_1592 mRNA_1593
9957 9958 9959 9960 9961
facs on lipid accumulation Sorted No BODIPY Gate -1.6 -0.9 -0.3 -0.4 +0.2
facs on lipid accumulation Sorted Low BODIPY Gate -2.0 -0.4 -0.6 +0.1 +0.3
buoyancy on lipid accumulation 40 hrs Median Buoyancy -1.7 -0.5 -0.2 -0.3 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.8 -0.3 -0.4 -0.2 -0.9
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -1.8 -0.1 +0.0 -0.4 +0.1
auxotrophy Dropout Complete -1.6 -0.3 -0.2 +0.0 +0.1
auxotrophy YNB Glucose 75mM Arginine -1.4 -0.4 -0.2 +0.2 -0.1
growth on oleic acid YNB Oleic Acid -1.2 -0.1 +0.1 -0.1 -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy -1.7 +0.3 -0.2 -0.2 +0.2
auxotrophy YNB Glucose 75mM Methionine -0.9 -0.1 -0.2 -0.2 +0.0
ypd buoyancy 40 hrs Low Buoyancy -0.5 +0.1 -0.9 -0.2 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy -1.0 -0.3 -0.1 +0.1 +0.1
auxotrophy YNB Glucose -1.7 +0.2 +0.2 +0.1 +0.0
facs on lipid accumulation Sorted High BODIPY Gate -0.9 -0.2 +0.1 +0.1 +0.0
growth on oleic acid YNB Glucose -1.0 +0.2 +0.2 -0.1 -0.2
growth on ricinoleic acid YNB Glucose -0.3 -0.1 +0.1 +0.1 -0.2
growth on ricinoleic acid YNB Methylricinoleic Acid +0.3 -0.2 -0.2 +0.2 -0.5
ypd buoyancy 40 hrs Median Buoyancy -1.3 +0.5 +0.6 -0.2 +0.4
ypd buoyancy 40 hrs No Buoyancy Separation -0.8 +0.4 +0.7 -0.4 +0.2
growth on ypd YPD +0.5 +0.4 -0.3 -0.1 +0.2
ypd buoyancy 40 hrs High Buoyancy -0.4 +0.9 +0.1 -0.4 +0.7
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