Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9959 and 9960 are separated by 550 nucleotides9960 and 9961 are separated by 1031 nucleotides9961 and 9962 are separated by 1221 nucleotides9962 and 9963 are separated by 308 nucleotides mRNA_1591: 9959 - K01835 pgm phosphoglucomutase, at 673,406 to 675,711 9959 mRNA_1592: 9960 - HMMPfam-bZIP transcription factor-PF00170,ProSitePatterns-Basic-leucine zipper (bZIP) domain signature.-PS00036,ProSiteProfiles-Basic-leucine zipper (bZIP) domain profile.-PS50217,SMART-basic region leucin zipper-SM00338,SUPERFAMILY--SSF57959, at 676,262 to 677,615 9960 mRNA_1593: 9961 - KOG4366 Predicted thioesterase, at 678,647 to 679,781 9961 mRNA_1594: 9962 - K16261 YAT yeast amino acid transporter, at 681,003 to 683,049 9962 mRNA_1595: 9963 - K13342 PEX5, PXR1 peroxin-5, at 683,358 to 685,744 9963
Group Condition mRNA_1591 mRNA_1592 mRNA_1593 mRNA_1594 mRNA_1595
9959 9960 9961 9962 9963
growth on ricinoleic acid YNB Ricinoleic Acid -0.4 -0.2 -0.9 -0.2 N.D.
ypd buoyancy 40 hrs Low Buoyancy -0.9 -0.2 +0.2 +0.1 N.D.
growth on oleic acid YNB Oleic Acid +0.1 -0.1 -0.4 -0.2 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid -0.2 +0.2 -0.5 -0.1 N.D.
growth on oleic acid YNB Glucose +0.2 -0.1 -0.2 -0.2 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate -0.6 +0.1 +0.3 +0.0 N.D.
auxotrophy YNB Glucose 75mM Methionine -0.2 -0.2 +0.0 +0.1 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.3 -0.4 +0.2 +0.3 N.D.
auxotrophy YNB Glucose 75mM Arginine -0.2 +0.2 -0.1 -0.0 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.3 +0.1 +0.4 N.D.
auxotrophy Dropout Complete -0.2 +0.0 +0.1 +0.0 N.D.
growth on ricinoleic acid YNB Glucose +0.1 +0.1 -0.2 +0.0 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.2 -0.2 +0.2 +0.4 N.D.
facs on lipid accumulation Sorted High BODIPY Gate +0.1 +0.1 +0.0 +0.0 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.0 -0.4 +0.1 +0.5 N.D.
ypd buoyancy 40 hrs No Buoyancy Separation +0.7 -0.4 +0.2 -0.3 N.D.
growth on ypd YPD -0.3 -0.1 +0.2 +0.4 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.1 -0.4 +0.7 -0.1 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy -0.1 +0.1 +0.1 +0.4 N.D.
auxotrophy YNB Glucose +0.2 +0.1 +0.0 +0.1 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.6 -0.2 +0.4 +0.1 N.D.
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