Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9969 and 9970 are separated by 277 nucleotides9970 and 9971 are separated by 492 nucleotides9971 and 9972 are separated by 143 nucleotides9972 and 9973 are separated by 94 nucleotides mRNA_1601: 9969 - HMMPfam-Protein of unknown function (DUF1682)-PF07946,ProSiteProfiles-Prokaryotic membrane lipoprotein lipid attachment site profile.-PS51257, at 694,292 to 695,992 9969 mRNA_1602: 9970 - HMMPfam-Protein of unknown function (DUF2013)-PF09431, at 696,270 to 698,823 9970 mRNA_1603: 9971 - KOG2948 Predicted metal-binding protein, at 699,316 to 700,594 9971 mRNA_1604: 9972 - K02209 MCM5, CDC46 DNA replication licensing factor MCM5, at 700,738 to 703,638 9972 mRNA_1605: 9973 - Hypothetical Protein, at 703,733 to 704,608 9973
Group Condition mRNA_1601 mRNA_1602 mRNA_1603 mRNA_1604 mRNA_1605
9969 9970 9971 9972 9973
facs on lipid accumulation Sorted High BODIPY Gate -0.4 -0.6 N.D. N.D. -0.1
growth on ricinoleic acid YNB Ricinoleic Acid -0.6 -0.2 N.D. N.D. -0.2
growth on oleic acid YNB Oleic Acid -0.4 -0.0 N.D. N.D. -0.2
buoyancy on lipid accumulation 40 hrs Median Buoyancy -0.2 -0.8 N.D. N.D. +0.3
growth on ypd YPD +0.0 -0.2 N.D. N.D. -0.4
facs on lipid accumulation Sorted Low BODIPY Gate -0.0 -0.1 N.D. N.D. -0.4
buoyancy on lipid accumulation 40 hrs High Buoyancy -0.4 -0.5 N.D. N.D. +0.5
auxotrophy Dropout Complete -0.1 +0.0 N.D. N.D. -0.1
facs on lipid accumulation Sorted No BODIPY Gate +0.1 -0.2 N.D. N.D. +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid -0.5 +0.5 N.D. N.D. -0.0
auxotrophy YNB Glucose 75mM Methionine -0.1 +0.3 N.D. N.D. -0.1
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation -0.1 -0.3 N.D. N.D. +0.6
auxotrophy YNB Glucose +0.2 +0.3 N.D. N.D. -0.3
auxotrophy YNB Glucose 75mM Arginine -0.0 +0.1 N.D. N.D. +0.2
ypd buoyancy 40 hrs No Buoyancy Separation +0.3 -0.2 N.D. N.D. +0.2
ypd buoyancy 40 hrs Median Buoyancy +0.3 +0.2 N.D. N.D. -0.1
growth on oleic acid YNB Glucose +0.1 +0.1 N.D. N.D. +0.4
growth on ricinoleic acid YNB Glucose +0.3 +0.7 N.D. N.D. -0.3
ypd buoyancy 40 hrs High Buoyancy +0.3 -0.1 N.D. N.D. +0.5
ypd buoyancy 40 hrs Low Buoyancy +0.3 +0.1 N.D. N.D. +0.5
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.1 +0.8 N.D. N.D. +0.4
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