Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9971 and 9972 are separated by 143 nucleotides9972 and 9973 are separated by 94 nucleotides9973 and 9974 are separated by 412 nucleotides9974 and 9975 are separated by 506 nucleotides mRNA_1603: 9971 - KOG2948 Predicted metal-binding protein, at 699,316 to 700,594 9971 mRNA_1604: 9972 - K02209 MCM5, CDC46 DNA replication licensing factor MCM5, at 700,738 to 703,638 9972 mRNA_1605: 9973 - Hypothetical Protein, at 703,733 to 704,608 9973 mRNA_1606: 9974 - Hypothetical Protein, at 705,021 to 705,665 9974 mRNA_1607: 9975 - HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 706,172 to 709,618 9975
Group Condition mRNA_1603 mRNA_1604 mRNA_1605 mRNA_1606 mRNA_1607
9971 9972 9973 9974 9975
ypd buoyancy 40 hrs No Buoyancy Separation N.D. N.D. +0.2 -3.2 -0.1
facs on lipid accumulation Sorted Low BODIPY Gate N.D. N.D. -0.4 -0.7 +0.0
buoyancy on lipid accumulation 40 hrs Median Buoyancy N.D. N.D. +0.3 -1.5 +0.2
buoyancy on lipid accumulation 40 hrs High Buoyancy N.D. N.D. +0.5 -1.3 +0.0
auxotrophy Dropout Complete N.D. N.D. -0.1 -0.7 +0.0
auxotrophy YNB Glucose N.D. N.D. -0.3 -0.4 +0.2
buoyancy on lipid accumulation 40 hrs Low Buoyancy N.D. N.D. +0.4 -1.4 +0.4
auxotrophy YNB Glucose 75mM Methionine N.D. N.D. -0.1 -0.3 +0.1
growth on oleic acid YNB Oleic Acid N.D. N.D. -0.2 -0.1 +0.1
growth on ricinoleic acid YNB Ricinoleic Acid N.D. N.D. -0.2 -0.0 +0.1
ypd buoyancy 40 hrs Median Buoyancy N.D. N.D. -0.1 -0.3 +0.3
growth on ricinoleic acid YNB Glucose N.D. N.D. -0.3 +0.3 +0.0
auxotrophy YNB Glucose 75mM Arginine N.D. N.D. +0.2 -0.3 +0.0
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation N.D. N.D. +0.6 -0.7 +0.1
growth on ricinoleic acid YNB Methylricinoleic Acid N.D. N.D. -0.0 +0.1 +0.1
ypd buoyancy 40 hrs High Buoyancy N.D. N.D. +0.5 -0.3 +0.0
facs on lipid accumulation Sorted High BODIPY Gate N.D. N.D. -0.1 +0.5 -0.2
facs on lipid accumulation Sorted No BODIPY Gate N.D. N.D. +0.1 +0.4 -0.2
ypd buoyancy 40 hrs Low Buoyancy N.D. N.D. +0.5 -0.2 +0.2
growth on ypd YPD N.D. N.D. -0.4 +0.8 +0.1
growth on oleic acid YNB Glucose N.D. N.D. +0.4 +0.6 +0.0
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