Fitness for 5 genes in Rhodosporidium toruloides IFO0880

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500 nt 9975 and 9976 are separated by 319 nucleotides9976 and 9977 are separated by 1127 nucleotides9977 and 9978 are separated by 1196 nucleotides9978 and 9979 are separated by 144 nucleotides mRNA_1607: 9975 - HMMPfam-Fungal specific transcription factor domain-PF04082,ProSitePatterns-Zn(2)-C6 fungal-type DNA-binding domain signature.-PS00463,ProSiteProfiles-Zn(2)-C6 fungal-type DNA-binding domain profile.-PS50048,SMART-GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain-SM00066,SMART-Fungal specific transcription factor domain-SM00906,SUPERFAMILY--SSF57701, at 706,172 to 709,618 9975 mRNA_1608: 9976 - K19704 PTC1 protein phosphatase PTC1, at 709,938 to 711,637 9976 mRNA_1609: 9977 - HMMPfam-Ferritin-like domain-PF13668,SUPERFAMILY--SSF47240, at 712,765 to 713,275 9977 mRNA_1610: 9978 - HMMPfam-CrcB-like protein-PF02537, at 714,472 to 715,757 9978 mRNA_1611: 9979 - K04708 E1.1.1.102 3-dehydrosphinganine reductase, at 715,902 to 717,266 9979
Group Condition mRNA_1607 mRNA_1608 mRNA_1609 mRNA_1610 mRNA_1611
9975 9976 9977 9978 9979
ypd buoyancy 40 hrs No Buoyancy Separation -0.1 -1.2 -0.1 -1.3 N.D.
auxotrophy Dropout Complete +0.0 -0.6 -0.8 -0.6 N.D.
auxotrophy YNB Glucose 75mM Arginine +0.0 -0.2 -0.4 -0.8 N.D.
facs on lipid accumulation Sorted Low BODIPY Gate +0.0 +0.1 +0.0 -1.0 N.D.
auxotrophy YNB Glucose 75mM Methionine +0.1 -0.2 -0.4 -0.4 N.D.
buoyancy on lipid accumulation 40 hrs No Buoyancy Separation +0.1 +0.0 -0.5 -0.3 N.D.
buoyancy on lipid accumulation 40 hrs High Buoyancy +0.0 -0.0 +0.2 -0.7 N.D.
growth on ricinoleic acid YNB Methylricinoleic Acid +0.1 -0.0 -0.3 -0.1 N.D.
ypd buoyancy 40 hrs High Buoyancy +0.0 -0.2 -0.3 +0.1 N.D.
growth on ricinoleic acid YNB Ricinoleic Acid +0.1 -0.0 -0.5 +0.0 N.D.
auxotrophy YNB Glucose +0.2 -0.5 +0.2 -0.1 N.D.
growth on oleic acid YNB Oleic Acid +0.1 -0.3 -0.2 +0.3 N.D.
facs on lipid accumulation Sorted No BODIPY Gate -0.2 +0.6 -0.0 -0.5 N.D.
buoyancy on lipid accumulation 40 hrs Median Buoyancy +0.2 +0.1 +0.2 -0.6 N.D.
growth on oleic acid YNB Glucose +0.0 -0.0 -0.3 +0.3 N.D.
ypd buoyancy 40 hrs Median Buoyancy +0.3 -0.0 -0.1 -0.0 N.D.
facs on lipid accumulation Sorted High BODIPY Gate -0.2 +0.4 +0.1 -0.1 N.D.
ypd buoyancy 40 hrs Low Buoyancy +0.2 -0.2 -0.0 +0.1 N.D.
growth on ypd YPD +0.1 +0.8 -0.2 -0.4 N.D.
growth on ricinoleic acid YNB Glucose +0.0 -0.2 -0.4 +0.9 N.D.
buoyancy on lipid accumulation 40 hrs Low Buoyancy +0.4 +0.3 +0.3 -0.1 N.D.
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