MetaCyc Pathway: adenosylcobalamin biosynthesis II (aerobic) in Rhodosporidium toruloides IFO0880

Add experiment(s):


Glutamate--tRNA ligase:
L-glutamate + ATP + a tRNAglu→an L-glutamyl-[tRNAGlu] + AMP + diphosphate
(EC 6.1.1.17)
mRNA_2385 (10753)
mRNA_6197 (14565)
Riboflavin kinase:
riboflavin + ATP→FMN + ADP + H+
(EC 2.7.1.26)
mRNA_930 (9298)
L-threonine + ATP→L-threonine 3-O-phosphate + ADP + H+ (EC 2.7.1.177)
No genes
Threonine-phosphate decarboxylase:
L-threonine 3-O-phosphate + H+→(R)-1-amino-2-propanol O-2-phosphate + CO2
(EC 4.1.1.81)
No genes
FMN + NAD(P)H + 2 H+→FMNH2 + NAD(P)+ (EC 1.5.1.39)
No genes
Glutamyl-tRNA reductase (in reverse):
an L-glutamyl-[tRNAGlu] + NADPH + H+→(S)-4-amino-5-oxopentanoate + a tRNAglu + NADP+
(EC 1.2.1.70)
No genes
Glutamate-1-semialdehyde 2,1-aminomutase:
(S)-4-amino-5-oxopentanoate→5-aminolevulinate
(EC 5.4.3.8)
No genes
FMNH2 + dioxygen→5,6-dimethylbenzimidazole + dialurate + D-erythrose 4-phosphate (EC 1.13.11.79)
No genes
Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase:
5,6-dimethylbenzimidazole + β-nicotinate D-ribonucleotide→α-ribazole 5'-phosphate + nicotinate + H+
(EC 2.4.2.21)
No genes
Porphobilinogen synthase:
2 5-aminolevulinate→porphobilinogen + H+ + 2 H2O
(EC 4.2.1.24)
mRNA_5026 (13394)
Hydroxymethylbilane synthase:
4 porphobilinogen + H2O→preuroporphyrinogen + 4 ammonium
(EC 2.5.1.61)
mRNA_6350 (14718)
Uroporphyrinogen-III synthase:
preuroporphyrinogen→uroporphyrinogen-III + H2O
(EC 4.2.1.75)
No genes
Uroporphyrinogen-III C-methyltransferase:
uroporphyrinogen-III + S-adenosyl-L-methionine→precorrin-1 + S-adenosyl-L-homocysteine + H+
(EC 2.1.1.107)
No genes
Uroporphyrinogen-III C-methyltransferase:
precorrin-1 + S-adenosyl-L-methionine→precorrin-2 + S-adenosyl-L-homocysteine
(EC 2.1.1.107)
No genes
Precorrin-2 C(20)-methyltransferase:
precorrin-2 + S-adenosyl-L-methionine→precorrin-3A + S-adenosyl-L-homocysteine + H+
(EC 2.1.1.130)
No genes
Precorrin-3B synthase:
precorrin-3A + NADH + dioxygen + H+→precorrin-3B + NAD+ + H2O
(EC 1.14.13.83)
No genes
Precorrin-3B C(17)-methyltransferase:
precorrin-3B + S-adenosyl-L-methionine→precorrin-4 + S-adenosyl-L-homocysteine + 2 H+
(EC 2.1.1.131)
No genes
Precorrin-4 C(11)-methyltransferase:
precorrin-4 + S-adenosyl-L-methionine→precorrin-5 + S-adenosyl-L-homocysteine + H+
(EC 2.1.1.133)
No genes
Precorrin-6A synthase (deacetylating):
precorrin-5 + S-adenosyl-L-methionine + H2O→precorrin-6A + acetate + S-adenosyl-L-homocysteine + 2 H+
(EC 2.1.1.152)
No genes
Precorrin-6A reductase (in reverse):
precorrin-6A + NADPH + H+→precorrin-6B + NADP+
(EC 1.3.1.54)
No genes
Precorrin-6Y C(5,15)-methyltransferase (decarboxylating):
precorrin-6B + H+ + S-adenosyl-L-methionine→precorrin-7 + S-adenosyl-L-homocysteine + CO2
(EC 2.1.1.132)
No genes
Precorrin-6Y C(5,15)-methyltransferase (decarboxylating):
precorrin-7 + S-adenosyl-L-methionine→precorrin-8x + S-adenosyl-L-homocysteine + 2 H+
(EC 2.1.1.132)
No genes
precorrin-8x + H+→hydrogenobyrinate (EC 5.4.99.61)
No genes
Hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolyzing):
hydrogenobyrinate + 2 ATP + 2 L-glutamine + 2 H2O→hydrogenobyrinate a,c-diamide + 2 L-glutamate + 2 ADP + 2 H+ + 2 phosphate
(EC 6.3.5.9)
No genes
Cobaltochelatase:
hydrogenobyrinate a,c-diamide + ATP + Co2+ + H2O→cob(II)yrinate a,c-diamide + ADP + 3 H+ + phosphate
(EC 6.6.1.2)
No genes
Cob(I)yrinic acid a,c-diamide adenosyltransferase:
cob(II)yrinate a,c-diamide + a [corrinoid adenosyltransferase]→cob(II)yrinate a,c-diamide-[corrinoid adenosyltranferase]
(EC 2.5.1.17)
No genes
Cob(I)yrinic acid a,c-diamide adenosyltransferase:
2 cob(II)yrinate a,c-diamide-[corrinoid adenosyltranferase] + a [reduced flavoprotein]→2 cob(I)yrinate a,c-diamide-[corrinoid adenosyltranferase] + an [oxidized flavoprotein] + 2 H+
(spontaneous) (EC 2.5.1.17)
No genes
Cob(I)yrinic acid a,c-diamide adenosyltransferase:
2 cob(I)yrinate a,c-diamide-[corrinoid adenosyltranferase] + 2 ATP→2 adenosyl-cob(III)yrinate a,c-diamide + 2 a [corrinoid adenosyltransferase] + 2 triphosphate
(EC 2.5.1.17)
No genes
Adenosylcobyric acid synthase (glutamine-hydrolyzing):
adenosyl-cob(III)yrinate a,c-diamide + 4 ATP + 4 L-glutamine + 4 H2O→adenosylcobyrate + 4 L-glutamate + 4 ADP + 4 H+ + 4 phosphate
(EC 6.3.5.10)
No genes
Adenosylcobinamide-phosphate synthase:
adenosylcobyrate + (R)-1-amino-2-propanol O-2-phosphate + ATP→adenosyl-cobinamide phosphate + ADP + H+ + phosphate
(EC 6.3.1.10)
No genes
Adenosylcobinamide-phosphate guanylyltransferase:
adenosyl-cobinamide phosphate + GTP + H+→adenosylcobinamide-GDP + diphosphate
(EC 2.7.7.62)
No genes
Adenosylcobinamide-GDP ribazoletransferase:
adenosylcobinamide-GDP + α-ribazole 5'-phosphate→adenosylcobalamin 5'-phosphate + GMP + H+
(EC 2.7.8.26)
No genes
Alpha-ribazole phosphatase:
adenosylcobalamin 5'-phosphate + H2O→adenosylcobalamin + phosphate
(EC 3.1.3.73)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information