MetaCyc Pathway: L-isoleucine biosynthesis III in Rhodosporidium toruloides IFO0880

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Methylaspartate mutase (in reverse):
L-glutamate→(2S, 3S)-3-methylaspartate (EC 5.4.99.1)
No genes
(2S, 3S)-3-methylaspartate + 2-oxoglutarate→L-glutamate + methyloxaloacetate
No genes
methyloxaloacetate + H+→2-oxobutanoate + CO2
No genes
Acetolactate synthase:
2-oxobutanoate + H+ + pyruvate→(S)-2-aceto-2-hydroxybutanoate + CO2 (EC 2.2.1.6)
mRNA_7317 (15685)
mRNA_1432 (9800)
Ketol-acid reductoisomerase (in reverse):
(S)-2-aceto-2-hydroxybutanoate + NADPH + H+→(R)-2,3-dihydroxy-3-methylpentanoate + NADP+ (EC 1.1.1.86)
mRNA_808 (9176)
mRNA_809 (9177)
mRNA_810 (9178)
Dihydroxy-acid dehydratase:
(R)-2,3-dihydroxy-3-methylpentanoate→(3S)-3-methyl-2-oxopentanoate + H2O (EC 4.2.1.9)
mRNA_6258 (14626)
mRNA_1321 (9689)
Branched-chain-amino-acid transaminase (in reverse):
(3S)-3-methyl-2-oxopentanoate + L-glutamate→L-isoleucine + 2-oxoglutarate (EC 2.6.1.42)
mRNA_5646 (14014)
mRNA_6242 (14610)
mRNA_6485 (14853)

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information