MetaCyc Pathway: ajmaline and sarpagine biosynthesis in Rhodosporidium toruloides IFO0880

Add experiment(s):


3-alpha-(S)-strictosidine beta-glucosidase:
3-α(S)-strictosidine + H2O→strictosidine aglycone + D-glucopyranose (EC 3.2.1.105)
No genes
strictosidine aglycone→cathenamine + H2O (spontaneous)
No genes
cathenamine→4,21-dehydrogeissoschizine (spontaneous)
No genes
Geissoschizine dehydrogenase (in reverse):
4,21-dehydrogeissoschizine + NADPH→19E-geissoschizine + NADP+ (EC 1.3.1.36)
No genes
19E-geissoschizine + dioxygen + a reduced [NADPH-hemoprotein reductase]→polyneuridine aldehyde + an oxidized [NADPH-hemoprotein reductase] + 2 H2O (EC 1.14.19.M24)
No genes
Polyneuridine-aldehyde esterase:
polyneuridine aldehyde + H2O→16-epivellosimine + CO2 + methanol (EC 3.1.1.78)
No genes
Vinorine synthase:
16-epivellosimine + acetyl-CoA→vinorine + coenzyme A (EC 2.3.1.160)
No genes
16-epivellosimine→vellosimine (spontaneous)
No genes
Vellosimine dehydrogenase (in reverse):
vellosimine + NADPH + H+→10-deoxysarpagine + NADP+ (EC 1.1.1.273)
No genes
vinorine + dioxygen + a reduced [NADPH-hemoprotein reductase]→21-epi-vomilenine + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.104)
No genes
10-deoxysarpagine + dioxygen + a reduced [NADPH-hemoprotein reductase]→sarpagine + an oxidized [NADPH-hemoprotein reductase] + H2O (EC 1.14.14.136)
No genes
21-epi-vomilenine→vomilenine (spontaneous)
No genes
Vomilenine reductase (in reverse):
vomilenine + NADPH + H+→1,2-dihydrovomilenine + NADP+ (EC 1.5.1.32)
No genes
Vomilenine glucosyltransferase:
vomilenine + UDP-α-D-glucose→raucaffricine + H+ + UDP (EC 2.4.1.219)
No genes
vomilenine + dioxygen + 2 a reduced [NADPH-hemoprotein reductase]→perakine + 2 an oxidized [NADPH-hemoprotein reductase] + 2 H2O (EC 1.14.14.104)
No genes
vomilenine + NADPH + H+→(20αS)-19,20--dihydrovomilenine + NADP+ (EC 1.3.1.M19)
No genes
1,2-dihydrovomilenine reductase (in reverse):
1,2-dihydrovomilenine + NADPH + H+→17-O-acetylnorajmaline + NADP+ (EC 1.3.1.73)
No genes
perakine + NADPH + H+→raucaffrinoline + NADP+ (EC 1.1.1.317)
mRNA_6001 (14369)
(20αS)-19,20--dihydrovomilenine + NADPH + H+→17-O-acetylnorajmaline + NADP+
No genes
17-O-acetylnorajmaline + S-adenosyl-L-methionine→17-O-acetylajmaline + S-adenosyl-L-homocysteine + H+
No genes
Acetylajmaline esterase:
17-O-acetylnorajmaline + H2O→norajmaline + acetate + H+ (EC 3.1.1.80)
No genes
norajmaline + S-adenosyl-L-methionine→ajmaline + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.M48)
No genes
Acetylajmaline esterase:
17-O-acetylajmaline + H2O→ajmaline + acetate + H+ (EC 3.1.1.80)
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information