MetaCyc Pathway: adenosylcobalamin biosynthesis I (anaerobic) in Rhodosporidium toruloides IFO0880

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Glutamate--tRNA ligase:
L-glutamate + ATP + a tRNAglu→an L-glutamyl-[tRNAGlu] + AMP + diphosphate
(EC 6.1.1.17)
mRNA_2385 (10753)
mRNA_6197 (14565)
5-amino-1-(5-phospho-β-D-ribosyl)imidazole + a reduced unknown two electron carrier + S-adenosyl-L-methionine→5-hydroxybenzimidazole + ammonium + an oxidized unknown electron carrier + 5'-deoxyadenosine + formate + L-methionine + 2 H+ + phosphate (EC 4.1.99.23)
No genes
L-threonine + ATP→L-threonine 3-O-phosphate + ADP + H+ (EC 2.7.1.177)
No genes
Threonine-phosphate decarboxylase:
L-threonine 3-O-phosphate + H+→(R)-1-amino-2-propanol O-2-phosphate + CO2
(EC 4.1.1.81)
No genes
Glutamyl-tRNA reductase (in reverse):
an L-glutamyl-[tRNAGlu] + NADPH + H+→(S)-4-amino-5-oxopentanoate + a tRNAglu + NADP+
(EC 1.2.1.70)
No genes
5-hydroxybenzimidazole + S-adenosyl-L-methionine→5-methoxybenzimidazole + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.M9)
No genes
Glutamate-1-semialdehyde 2,1-aminomutase:
(S)-4-amino-5-oxopentanoate→5-aminolevulinate
(EC 5.4.3.8)
No genes
5-methoxybenzimidazole + S-adenosyl-L-methionine→5-methoxy-6-methylbenzimidazole + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.M10)
No genes
Porphobilinogen synthase:
2 5-aminolevulinate→porphobilinogen + H+ + 2 H2O
(EC 4.2.1.24)
mRNA_5026 (13394)
5-methoxy-6-methylbenzimidazole→5,6-dimethylbenzimidazole
No genes
Nicotinate-nucleotide--dimethylbenzimidazole phosphoribosyltransferase:
5,6-dimethylbenzimidazole + β-nicotinate D-ribonucleotide→α-ribazole 5'-phosphate + nicotinate + H+
(EC 2.4.2.21)
No genes
Hydroxymethylbilane synthase:
4 porphobilinogen + H2O→preuroporphyrinogen + 4 ammonium
(EC 2.5.1.61)
mRNA_6350 (14718)
Uroporphyrinogen-III synthase:
preuroporphyrinogen→uroporphyrinogen-III + H2O
(EC 4.2.1.75)
No genes
Uroporphyrinogen-III C-methyltransferase:
uroporphyrinogen-III + S-adenosyl-L-methionine→precorrin-1 + S-adenosyl-L-homocysteine + H+
(EC 2.1.1.107)
No genes
Uroporphyrinogen-III C-methyltransferase:
precorrin-1 + S-adenosyl-L-methionine→precorrin-2 + S-adenosyl-L-homocysteine
(EC 2.1.1.107)
No genes
Precorrin-2 dehydrogenase:
precorrin-2 + NAD+→sirohydrochlorin + NADH + 2 H+
(EC 1.3.1.76)
No genes
Sirohydrochlorin cobaltochelatase:
sirohydrochlorin + Co2+→cobalt-sirohydrochlorin + 2 H+
(EC 4.99.1.3)
No genes
Cobalt-factor II C(20)-methyltransferase:
cobalt-sirohydrochlorin + S-adenosyl-L-methionine→cobalt-factor III + S-adenosyl-L-homocysteine + H+
(EC 2.1.1.151)
No genes
cobalt-factor III + a reduced unknown two electron carrier→cobalt-precorrin-3 + an oxidized unknown electron carrier (EC 2.1.1.272)
No genes
cobalt-precorrin-3 + S-adenosyl-L-methionine→cobalt-precorrin-4 + S-adenosyl-L-homocysteine (EC 2.1.1.272)
No genes
cobalt-precorrin-4 + S-adenosyl-L-methionine→cobalt-precorrin-5A + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.271)
No genes
cobalt-precorrin-5A + H2O→cobalt-precorrin-5B + acetaldehyde + H+ (EC 3.7.1.12)
No genes
cobalt-precorrin-5B + S-adenosyl-L-methionine→cobalt-precorrin-6A + S-adenosyl-L-homocysteine (EC 2.1.1.195)
No genes
cobalt-precorrin-6A + NADH→cobalt-precorrin-6B + NAD+ (EC 1.3.1.106)
No genes
cobalt-precorrin-6B + H+ + S-adenosyl-L-methionine→cobalt-precorrin-7 + S-adenosyl-L-homocysteine + CO2 (EC 2.1.1.196)
No genes
cobalt-precorrin-7 + S-adenosyl-L-methionine→cobalt-precorrin-8 + S-adenosyl-L-homocysteine (EC 2.1.1.289)
No genes
cobalt-precorrin-8→cobyrinate + H+ (EC 5.4.99.60)
No genes
cobyrinate + 2 ATP + 2 L-glutamine + 2 H2O→cob(II)yrinate a,c-diamide + 2 L-glutamate + 2 ADP + 2 H+ + 2 phosphate (EC 6.3.5.11)
No genes
Cob(I)yrinic acid a,c-diamide adenosyltransferase:
cob(II)yrinate a,c-diamide + a [corrinoid adenosyltransferase]→cob(II)yrinate a,c-diamide-[corrinoid adenosyltranferase]
(EC 2.5.1.17)
No genes
Cob(I)yrinic acid a,c-diamide adenosyltransferase:
2 cob(II)yrinate a,c-diamide-[corrinoid adenosyltranferase] + a [reduced flavoprotein]→2 cob(I)yrinate a,c-diamide-[corrinoid adenosyltranferase] + an [oxidized flavoprotein] + 2 H+
(spontaneous) (EC 2.5.1.17)
No genes
Cob(I)yrinic acid a,c-diamide adenosyltransferase:
2 cob(I)yrinate a,c-diamide-[corrinoid adenosyltranferase] + 2 ATP→2 adenosyl-cob(III)yrinate a,c-diamide + 2 a [corrinoid adenosyltransferase] + 2 triphosphate
(EC 2.5.1.17)
No genes
Adenosylcobyric acid synthase (glutamine-hydrolyzing):
adenosyl-cob(III)yrinate a,c-diamide + 4 ATP + 4 L-glutamine + 4 H2O→adenosylcobyrate + 4 L-glutamate + 4 ADP + 4 H+ + 4 phosphate
(EC 6.3.5.10)
No genes
Adenosylcobinamide-phosphate synthase:
adenosylcobyrate + (R)-1-amino-2-propanol O-2-phosphate + ATP→adenosyl-cobinamide phosphate + ADP + H+ + phosphate
(EC 6.3.1.10)
No genes
Adenosylcobinamide-phosphate guanylyltransferase:
adenosyl-cobinamide phosphate + GTP + H+→adenosylcobinamide-GDP + diphosphate
(EC 2.7.7.62)
No genes
Adenosylcobinamide-GDP ribazoletransferase:
adenosylcobinamide-GDP + α-ribazole 5'-phosphate→adenosylcobalamin 5'-phosphate + GMP + H+
(EC 2.7.8.26)
No genes
Alpha-ribazole phosphatase:
adenosylcobalamin 5'-phosphate + H2O→adenosylcobalamin + phosphate
(EC 3.1.3.73)
No genes

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information