3-beta-hydroxy-Delta(5)-steroid dehydrogenase:
sitosterol + NAD+→3-oxo-24-ethyl-cholest-5-ene + NADH + H+ (EC 1.1.1.145) |
| No genes |
Steroid Delta-isomerase:
3-oxo-24-ethyl-cholest-5-ene→3-oxo-24-ethyl-cholest-4-ene (EC 5.3.3.1) |
| No genes |
| 3-oxo-24-ethyl-cholest-4-ene + NADH + dioxygen + H+→3-oxo-24-ethyl-26-hydroxy-cholest-4-ene + NAD+ + H2O |
| No genes |
| 3-oxo-24-ethyl-26-hydroxy-cholest-4-ene + NAD+→3-oxo-24-ethyl-26-al-cholest-4-ene + NADH + H+ |
| No genes |
| 3-oxo-24-ethyl-26-al-cholest-4-ene + NAD+ + H2O→3-oxo-24-ethyl-cholest-4-en-26-oate + NADH + 2 H+ |
| No genes |
| 3-oxo-24-ethyl-cholest-4-en-26-oate + ATP + coenzyme A→3-oxo-24-ethyl-cholest-4-en-26-oyl-CoA + AMP + diphosphate |
| No genes |
| 3-oxo-24-ethyl-cholest-4-en-26-oyl-CoA + an oxidized unknown electron carrier→3-oxo-24-ethyl-cholest-4,24-dien-26-oyl-CoA + a reduced unknown two electron carrier |
| No genes |
| 3-oxo-24-ethyl-cholest-4,24-dien-26-oyl-CoA + ATP + hydrogencarbonate→3-oxo-24-(isopropanoyl)-cholest-4,24-dien-26-oyl-CoA + ADP + H+ + phosphate |
| No genes |
| 3-oxo-24-(isopropanoyl)-cholest-4,24-dien-26-oyl-CoA + H2O→3,24-dioxo-cholest-4-en-26-oate + propanoyl-CoA |
| No genes |
| 3,24-dioxo-cholest-4-en-26-oate + ATP + coenzyme A→3,24-dioxocholest-4-en-26-oyl-CoA + AMP + diphosphate |
| No genes |
Propanoyl-CoA C-acyltransferase (in reverse):
3,24-dioxocholest-4-en-26-oyl-CoA + coenzyme A→propanoyl-CoA + 3-oxochol-4-en-24-oyl-CoA (EC 2.3.1.176) |
| mRNA_5687 (14055) |
| 3-oxochol-4-en-24-oyl-CoA + an oxidized unknown electron carrier→3-oxochol-4,22-dien-24-oyl-CoA + a reduced unknown two electron carrier |
| No genes |
| 3-oxochol-4,22-dien-24-oyl-CoA + H2O→22-hydroxy-3-oxochol-4-en-24-oyl-CoA |
| No genes |
| 22-hydroxy-3-oxochol-4-en-24-oyl-CoA + NAD+→3,22-dioxochol-4-en-24-oyl-CoA + NADH + H+ |
| No genes |
Acetyl-CoA C-acyltransferase (in reverse):
3,22-dioxochol-4-en-24-oyl-CoA + coenzyme A→acetyl-CoA + 3-oxo-23,24-bisnorchol-4-en-22-oyl-CoA (EC 2.3.1.16) |
| mRNA_5445 (13813) |
| mRNA_5687 (14055) |
| mRNA_6860 (15228) |
| mRNA_310 (8678) |
| mRNA_517 (8885) |
| mRNA_697 (9065) |
| 3-oxo-23,24-bisnorchol-4-en-22-oyl-CoA + an oxidized unknown electron carrier→3-oxo-23,24-bisnorchol-4,17(20)-dien-22-oyl-CoA + a reduced unknown two electron carrier |
| No genes |
| 3-oxo-23,24-bisnorchol-4,17(20)-dien-22-oyl-CoA + H2O→17-hydroxy-3-oxo-4-pregnane-20-carboxyl-CoA |
| No genes |
| 17-hydroxy-3-oxo-4-pregnane-20-carboxyl-CoA→propanoyl-CoA + androstenedione |
| No genes |
Links:
Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information