MetaCyc Pathway: pyruvate fermentation to isobutanol (engineered) in Rhodosporidium toruloides IFO0880

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Acetolactate synthase:
2 pyruvate + H+→(S)-2-acetolactate + CO2 (EC 2.2.1.6)
mRNA_7317 (15685)
mRNA_1432 (9800)
Ketol-acid reductoisomerase (in reverse):
(S)-2-acetolactate + NADPH + H+→(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ (EC 1.1.1.86)
mRNA_808 (9176)
mRNA_809 (9177)
mRNA_810 (9178)
Dihydroxy-acid dehydratase:
(2R)-2,3-dihydroxy-3-methylbutanoate→3-methyl-2-oxobutanoate + H2O (EC 4.2.1.9)
mRNA_6258 (14626)
mRNA_1321 (9689)
Branched-chain-2-oxoacid decarboxylase:
3-methyl-2-oxobutanoate + H+→isobutanal + CO2 (EC 4.1.1.72)
No genes
Alcohol dehydrogenase (in reverse):
isobutanal + NADH + H+→isobutanol + NAD+ (EC 1.1.1.1)
mRNA_1661 (10029)
mRNA_1713 (10081)
mRNA_3282 (11650)
mRNA_3297 (11665)
mRNA_3939 (12307)
mRNA_4452 (12820)
mRNA_4507 (12875)
mRNA_5059 (13427)
mRNA_5186 (13554)
mRNA_5194 (13562)
mRNA_5579 (13947)
mRNA_5740 (14108)
mRNA_5741 (14109)
mRNA_7070 (15438)
mRNA_7110 (15478)
mRNA_8149 (16517)
mRNA_733 (9101)

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information