MetaCyc Pathway: photosynthetic 3-hydroxybutanoate biosynthesis (engineered) in Rhodosporidium toruloides IFO0880

Add experiment(s):


2 H2O[in] + [cytosol] + 2 a plastoquinone + 4 H+[out]→2 a plastoquinol + dioxygen[in] + 4 H+[in] (EC 1.10.3.9)
No genes
a plastoquinol + 2 an oxidized plastocyanin[in] + 2 H+[out]→2 a reduced plastocyanin[in] + a plastoquinone + 4 H+[in] (EC 7.1.1.6)
No genes
a reduced plastocyanin[in] + [cytosol] + an oxidized ferredoxin [iron-sulfur] cluster[out]→a reduced ferredoxin [iron-sulfur] cluster[out] + an oxidized plastocyanin[in] (EC 1.97.1.12)
No genes
Ferredoxin--NADP(+) reductase:
2 a reduced ferredoxin [iron-sulfur] cluster[out] + NADP+[out] + H+[out]→NADPH[out] + 2 an oxidized ferredoxin [iron-sulfur] cluster[out]
(EC 1.18.1.2)
mRNA_8407 (16775)
Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (phosphorylating) (in reverse):
3-phospho-D-glyceroyl phosphate + NADPH + H+→D-glyceraldehyde 3-phosphate + NADP+ + phosphate
(EC 1.2.1.13)
No genes
Triose-phosphate isomerase:
D-glyceraldehyde 3-phosphate→glycerone phosphate
(EC 5.3.1.1)
mRNA_6808 (15176)
mRNA_7700 (16068)
mRNA_8276 (16644)
mRNA_932 (9300)
Fructose-bisphosphate aldolase (in reverse):
glycerone phosphate + D-glyceraldehyde 3-phosphate→β-D-fructofuranose 1,6-bisphosphate
(EC 4.1.2.13)
mRNA_7052 (15420)
Fructose-bisphosphate aldolase (in reverse):
glycerone phosphate + D-erythrose 4-phosphate→D-sedoheptulose 1,7-bisphosphate
(EC 4.1.2.13)
mRNA_7052 (15420)
Fructose-bisphosphatase:
β-D-fructofuranose 1,6-bisphosphate + H2O→β-D-fructofuranose 6-phosphate + phosphate
(EC 3.1.3.11)
mRNA_7055 (15423)
Sedoheptulose-bisphosphatase:
D-sedoheptulose 1,7-bisphosphate + H2O→D-sedoheptulose 7-phosphate + phosphate
(EC 3.1.3.37)
No genes
Transketolase:
D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate→D-ribose 5-phosphate + D-xylulose 5-phosphate
(EC 2.2.1.1)
mRNA_7263 (15631)
Transketolase (in reverse):
β-D-fructofuranose 6-phosphate + D-glyceraldehyde 3-phosphate→D-xylulose 5-phosphate + D-erythrose 4-phosphate
(EC 2.2.1.1)
mRNA_7263 (15631)
Ribose-5-phosphate isomerase:
D-ribose 5-phosphate→D-ribulose 5-phosphate
(EC 5.3.1.6)
mRNA_4920 (13288)
Ribulose-phosphate 3-epimerase (in reverse):
D-xylulose 5-phosphate→D-ribulose 5-phosphate
(EC 5.1.3.1)
mRNA_7896 (16264)
Phosphoribulokinase:
D-ribulose 5-phosphate + ATP→D-ribulose-1,5-bisphosphate + ADP + H+
(EC 2.7.1.19)
No genes
Ribulose-bisphosphate carboxylase (in reverse):
D-ribulose-1,5-bisphosphate + CO2 + H2O→2 3-phospho-D-glycerate + 2 H+
(EC 4.1.1.39)
No genes
3-phospho-D-glycerate→2-phospho-D-glycerate (EC 5.4.2.12)
mRNA_4025 (12393)
mRNA_1542 (9910)
Phosphoglycerate kinase:
3-phospho-D-glycerate + ATP→3-phospho-D-glyceroyl phosphate + ADP
(EC 2.7.2.3)
mRNA_7353 (15721)
Phosphopyruvate hydratase:
2-phospho-D-glycerate→H2O + phosphoenolpyruvate
(EC 4.2.1.11)
mRNA_4451 (12819)
mRNA_8034 (16402)
mRNA_669 (9037)
Pyruvate kinase (in reverse):
phosphoenolpyruvate + ADP + H+→pyruvate + ATP
(EC 2.7.1.40)
mRNA_2701 (11069)
mRNA_4634 (13002)
pyruvate + coenzyme A + NAD+→CO2 + acetyl-CoA + NADH (EC 1.2.1.104)
mRNA_5262 (13630)
mRNA_5354 (13722)
mRNA_5580 (13948)
mRNA_5758 (14126)
Acetyl-CoA C-acyltransferase:
2 acetyl-CoA→acetoacetyl-CoA + coenzyme A
(EC 2.3.1.16; 2.3.1.9)
mRNA_5445 (13813)
mRNA_5687 (14055)
mRNA_6860 (15228)
mRNA_310 (8678)
mRNA_517 (8885)
mRNA_697 (9065)
3-hydroxyacyl-CoA dehydrogenase (in reverse):
acetoacetyl-CoA + NADH + H+→(S)-3-hydroxybutanoyl-CoA + NAD+
(EC 1.1.1.35)
mRNA_2835 (11203)
Acetoacetyl-CoA reductase (in reverse):
acetoacetyl-CoA + NADPH + H+→(3R)-3-hydroxybutanoyl-CoA + NADP+
(EC 1.1.1.36)
No genes
(S)-3-hydroxybutanoyl-CoA + H2O→(S)-3-hydroxybutanoate + coenzyme A + H+
No genes
(3R)-3-hydroxybutanoyl-CoA + H2O→(R)-3-hydroxybutanoate + coenzyme A + H+
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information