MetaCyc Pathway: taxadiene biosynthesis (engineered) in Rhodosporidium toruloides IFO0880

Add experiment(s):


1-deoxy-D-xylulose-5-phosphate synthase:
D-glyceraldehyde 3-phosphate + pyruvate + H+→1-deoxy-D-xylulose 5-phosphate + CO2 (EC 2.2.1.7)
No genes
1-deoxy-D-xylulose-5-phosphate reductoisomerase (in reverse):
1-deoxy-D-xylulose 5-phosphate + NADPH + H+→2-C-methyl-D-erythritol 4-phosphate + NADP+ (EC 1.1.1.267)
No genes
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase:
2-C-methyl-D-erythritol 4-phosphate + CTP + H+→4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol + diphosphate (EC 2.7.7.60)
No genes
4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase:
4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol + ATP→2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol + ADP + H+ (EC 2.7.1.148)
No genes
2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase:
2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol→2-C-methyl-D-erythritol-2,4-cyclodiphosphate + CMP (EC 4.6.1.12)
No genes
2-C-methyl-D-erythritol-2,4-cyclodiphosphate + a reduced flavodoxin→(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + an oxidized flavodoxin + H+ + H2O (EC 1.17.7.3)
No genes
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→3-methylbut-3-en-1-yl diphosphate + 2 an oxidized ferredoxin [iron-sulfur] cluster + H2O (EC 1.17.7.4)
No genes
(E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→prenyl diphosphate + 2 an oxidized ferredoxin [iron-sulfur] cluster + H2O (EC 1.17.7.4)
No genes
Dimethylallyltranstransferase:
prenyl diphosphate + 3-methylbut-3-en-1-yl diphosphate→geranyl diphosphate + diphosphate (EC 2.5.1.1)
mRNA_4576 (12944)
mRNA_8135 (16503)
Isopentenyl-diphosphate Delta-isomerase:
3-methylbut-3-en-1-yl diphosphate→prenyl diphosphate (EC 5.3.3.2)
mRNA_7835 (16203)
Geranyltranstransferase:
geranyl diphosphate + 3-methylbut-3-en-1-yl diphosphate→(2E,6E)-farnesyl diphosphate + diphosphate (EC 2.5.1.10)
mRNA_4576 (12944)
mRNA_8135 (16503)
Farnesyltranstransferase:
(2E,6E)-farnesyl diphosphate + 3-methylbut-3-en-1-yl diphosphate→geranylgeranyl diphosphate + diphosphate (EC 2.5.1.29)
mRNA_8135 (16503)
Taxadiene synthase:
geranylgeranyl diphosphate→taxa-4,11-diene + diphosphate (EC 4.2.3.17)
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information