MetaCyc Pathway: complex N-linked glycan biosynthesis (vertebrates) in Rhodosporidium toruloides IFO0880

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Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase:
Man5GlcNAc2-[protein] (isomer 5A1,2) + UDP-N-acetyl-α-D-glucosamine→Man5GlcNAc3-[protein] + H+ + UDP
(EC 2.4.1.101)
No genes
Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase:
Man5GlcNAc3-[protein] + 2 H2O→Man3GlcNAc3-[protein] + 2 α-D-mannopyranose
(EC 3.2.1.114)
No genes
Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase:
Man3GlcNAc3-[protein] + UDP-N-acetyl-α-D-glucosamine→Man3GlcNAc4-[protein] + H+ + UDP
(EC 2.4.1.143)
No genes
Beta-1,4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase:
Man3GlcNAc4-[protein] + UDP-N-acetyl-α-D-glucosamine→a {β-D-GlcNAc-(1→2)-α-D-Man-(1→3)-[β-D-GlcNAc-(1→2)-α-D-Man-(1→6)]-[β-D-GlcNAc-(1→4)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc}-N-Asn-[protein] + H+ + UDP
(EC 2.4.1.144)
mRNA_6968 (15336)
Alpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase:
Man3GlcNAc4-[protein] + UDP-N-acetyl-α-D-glucosamine→a {β-D-GlcNAc-(1→2)-[β-D-GlcNAc-(1→4)]-α-D-Man-(1→3)-[β-D-GlcNAc-(1→2)-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc}-N-Asn-[protein] + H+ + UDP
(EC 2.4.1.145)
No genes
Glycoprotein 6-alpha-L-fucosyltransferase:
Man3GlcNAc4-[protein] + GDP-β-L-fucose→an N4-{β-D-GlcNAc-(1→2)-α-D-Man-(1→3)-[β-D-GlcNAc-(1→2)-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-[α-L-Fuc-(1→6)]-β-D-GlcNAc}-N-Asn-[protein] + GDP + H+
(EC 2.4.1.68)
No genes
Alpha-1,6-mannosyl-glycoprotein 6-beta-N-acetylglucosaminyltransferase:
a {β-D-GlcNAc-(1→2)-[β-D-GlcNAc-(1→4)]-α-D-Man-(1→3)-[β-D-GlcNAc-(1→2)-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc}-N-Asn-[protein] + UDP-N-acetyl-α-D-glucosamine→a {β-D-GlcNAc-(1→2)-[β-D-GlcNAc-(1→4)]-α-D-Man-(1→3)-[β-D-GlcNAc-(1→2)-[β-D-GlcNAc-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc}-N-Asn-[protein] + H+ + UDP
(EC 2.4.1.155)
No genes
Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase:
a {β-D-GlcNAc-(1→2)-[β-D-GlcNAc-(1→4)]-α-D-Man-(1→3)-[β-D-GlcNAc-(1→2)-[β-D-GlcNAc-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc}-N-Asn-[protein] + 4 UDP-α-D-galactose→a {β-D-Gal-(1→4)-β-D-GlcNAc-(1→2)-[β-D-Gal-(1→4)-β-D-GlcNAc-(1→4)]-α-D-Man-(1→3)-[β-D-Gal-(1→4)-β-D-GlcNAc-(1→2)-[β-D-Gal-(1→4)-β-D-GlcNAc-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc}-N-Asn-[protein] + 4 H+ + 4 UDP
(EC 2.4.1.38)
No genes
N-acetyllactosaminide alpha-2,3-sialyltransferase:
a {β-D-Gal-(1→4)-β-D-GlcNAc-(1→2)-[β-D-Gal-(1→4)-β-D-GlcNAc-(1→4)]-α-D-Man-(1→3)-[β-D-Gal-(1→4)-β-D-GlcNAc-(1→2)-[β-D-Gal-(1→4)-β-D-GlcNAc-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc}-N-Asn-[protein] + CMP-N-acetyl-β-neuraminate→a {β-D-Gal-(1→4)-β-D-GlcNAc-(1→2)-[β-D-Gal-(1→4)-β-D-GlcNAc-(1→4)]-α-D-Man-(1→3)-[α-D-Sial-(2→3)-β-D-Gal-(1→4)-β-D-GlcNAc-(1→2)-[β-D-Gal-(1→4)-β-D-GlcNAc-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc}-N-Asn-[protein] + CMP + H+
(EC 2.4.99.6)
No genes
Beta-galactoside alpha-2,6-sialyltransferase:
a {β-D-Gal-(1→4)-β-D-GlcNAc-(1→2)-[β-D-Gal-(1→4)-β-D-GlcNAc-(1→4)]-α-D-Man-(1→3)-[α-D-Sial-(2→3)-β-D-Gal-(1→4)-β-D-GlcNAc-(1→2)-[β-D-Gal-(1→4)-β-D-GlcNAc-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc}-N-Asn-[protein] + CMP-N-acetyl-β-neuraminate→a {α-D-Sial-(2→6)-β-D-Gal-(1→4)-β-D-GlcNAc-(1→2)-[β-D-Gal-(1→4)-β-D-GlcNAc-(1→4)]-α-D-Man-(1→3)-[α-D-Sial-(2→3)-β-D-Gal-(1→4)-β-D-GlcNAc-(1→2)-[β-D-Gal-(1→4)-β-D-GlcNAc-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc}-N-Asn-[protein] + CMP + H+
(EC 2.4.99.1)
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information