MetaCyc Pathway: mupirocin biosynthesis in Rhodosporidium toruloides IFO0880

Add experiment(s):


a malonyl-[acp] + H+→an acetyl-[acp] + CO2 (EC 4.1.1.M15)
No genes
[Acyl-carrier-protein] S-malonyltransferase:
malonyl-CoA + a soluble [acyl-carrier protein]→a malonyl-[acp] + coenzyme A (EC 2.3.1.39)
mRNA_8174 (16542)
mRNA_409 (8777)
3 a malonyl-[acp] + 3-hydroxypropanoyl-[acp] + 6 NADPH + 9 H+→9-hydroxynonanoyl-[acp] + 3 a soluble [acyl-carrier protein] + 3 CO2 + 6 NADP+ + 3 H2O (EC 2.3.1.M63)
No genes
an acetyl-[acp] + 4 a malonyl-[acp] + 5 NADPH + 7 H+ + 2 S-adenosyl-L-methionine + a soluble holo-[acyl-carrier protein]→a (3S,4R,6E,8R,9S)-3,9-dihydroxy-4,8-dimethyldec-6-enoyl-[acp] + 5 a soluble [acyl-carrier protein] + 2 S-adenosyl-L-homocysteine + 4 CO2 + 5 NADP+ + 2 H2O (EC 2.3.1.M60)
No genes
2 a malonyl-[acp] + a (3S,4R,6E,8R,9S)-3,9-dihydroxy-4,8-dimethyldec-6-enoyl-[acp] + NADPH + 3 H+→(5R,7S,8R,10E,12R,13S)-5,7,13-trihydroxy-8,12-dimethyl-3-oxotetradec-10-enoyl-[acp] + 2 a soluble [acyl-carrier protein] + 2 CO2 + NADP+ (EC 2.3.1.M61)
No genes
(5R,7S,8R,10E,12R,13S)-5,7,13-trihydroxy-8,12-dimethyl-3-oxotetradec-10-enoyl-[acp] + NADPH + dioxygen + H+→(5S,6S,7R,8R,10E,12R,13S)-5,6,7,13-tetrahydroxy-8,12-dimethyl-3-oxotetradec-10-enoyl-[acp] + NADP+ + H2O (EC 1.14.13.M68)
No genes
an acetyl-[acp] + (5S,6S,7R,8R,10E,12R,13S)-5,6,7,13-tetrahydroxy-8,12-dimethyl-3-oxotetradec-10-enoyl-[acp] + H2O→(5S,6S,7R,8R,10E,12R,13S)-3-(carboxymethyl)-3,5,6,7,13-pentahydroxy-8,12-dimethyltetradec-10-enoyl-[acp] + a soluble [acyl-carrier protein] + H+ (EC 2.3.1.M62)
No genes
(5S,6S,7R,8R,10E,12R,13S)-3-(carboxymethyl)-3,5,6,7,13-pentahydroxy-8,12-dimethyltetradec-10-enoyl-[acp]→(2Z,5S,6S,7R,8R,10E,12R,13S)-3-(carboxymethyl)-5,6,7,13-tetrahydroxy-8,12-dimethyltetradeca-2,10-dienoyl-[acp] + H2O (EC 4.2.1.M23)
No genes
(2Z,5S,6S,7R,8R,10E,12R,13S)-3-(carboxymethyl)-5,6,7,13-tetrahydroxy-8,12-dimethyltetradeca-2,10-dienoyl-[acp] + H+→(2E,5S,6S,7R,8R,10E,12R,13S)-5,6,7,13-tetrahydroxy-3,8,12-trimethyltetradeca-2,10-dienoyl-[acp] + CO2 (EC 4.1.1.M16)
No genes
(2E,5S,6S,7R,8R,10E,12R,13S)-5,6,7,13-tetrahydroxy-3,8,12-trimethyltetradeca-2,10-dienoyl-[acp] + NADPH + dioxygen + H+→(2E,5S,6S,7R,8R)-5,6,7-trihydroxy-9-{3-[(2S,3S)-3-hydroxybutan-2-yl]oxiran-2-yl}-3,8-dimethylnon-2-enoyl-[acp] + NADP+ + H2O (EC 1.14.13.M69)
No genes
(2E,5S,6S,7R,8R,10E,12R,13S)-5,6,7,13-tetrahydroxy-3,8,12-trimethyltetradeca-2,10-dienoyl-[acp] + dioxygen→(2E)-3-methyl-4-[(2S,3R,4S,5R)-3,4,5-trihydroxy-5-[(2E,4R,5S)-5-hydroxy-4-methylhex-2-en-1-yl]oxan-2-yl]but-2-enoyl-[acp] + H2O (EC 1.13.12.M4)
No genes
(2E,5S,6S,7R,8R)-5,6,7-trihydroxy-9-{3-[(2S,3S)-3-hydroxybutan-2-yl]oxiran-2-yl}-3,8-dimethylnon-2-enoyl-[acp] + dioxygen→(2E)-3-methyl-4-[(2S,3R,4S,5R)-3,4,5-trihydroxy-5-({3-[(2S,3S)-3-hydroxybutan-2-yl]oxiran-2-yl}methyl)oxan-2-yl]but-2-enoyl-[acp] + H2O (EC 1.13.12.M4)
No genes
9-hydroxynonanoyl-[acp] + (2E)-3-methyl-4-[(2S,3R,4S,5R)-3,4,5-trihydroxy-5-[(2E,4R,5S)-5-hydroxy-4-methylhex-2-en-1-yl]oxan-2-yl]but-2-enoyl-[acp] + H2O→deoxypseudomonate B + 2 a soluble [acyl-carrier protein] + H+ (EC 2.3.1.M63)
No genes
9-hydroxynonanoyl-[acp] + (2E)-3-methyl-4-[(2S,3R,4S,5R)-3,4,5-trihydroxy-5-({3-[(2S,3S)-3-hydroxybutan-2-yl]oxiran-2-yl}methyl)oxan-2-yl]but-2-enoyl-[acp] + H2O→pseudomonate B + 2 a soluble [acyl-carrier protein] + H+ (EC 2.3.1.M63)
No genes
deoxypseudomonate B + a soluble [acyl-carrier protein] + ATP→deoxypseudomonic acid B-[mAcpE] + AMP + diphosphate (EC 6.3.2.M13)
No genes
deoxypseudomonic acid B-[mAcpE] + dioxygen + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→deoxymupirocin P-[acp] + 2 an oxidized ferredoxin [iron-sulfur] cluster + 2 H2O (EC 1.14.15.M8)
No genes
pseudomonate B + a soluble [acyl-carrier protein] + ATP→pseudomonic acid B-[mAcpE] + AMP + diphosphate (EC 6.3.2.M13)
No genes
pseudomonic acid B-[mAcpE] + dioxygen + 2 H+ + 2 a reduced ferredoxin [iron-sulfur] cluster→mupirocin P-[acp] + 2 an oxidized ferredoxin [iron-sulfur] cluster + 2 H2O (EC 1.14.15.M8)
No genes
deoxymupirocin P-[acp] + H2O→deoxymupirocin P + a soluble [acyl-carrier protein] + H+ (EC 3.1.2.M5)
No genes
mupirocin P-[acp] + H2O→mupirocin P + a soluble [acyl-carrier protein] + H+ (EC 3.1.2.M5)
No genes
deoxymupirocin P→deoxymupirocin C + H2O (EC 4.2.1.M24)
No genes
mupirocin P→mupirocin C1 + H2O (EC 4.2.1.M24)
No genes
deoxymupirocin C + NADPH + H+→deoxymupirocin F + NADP+ (EC 1.1.1.M50)
No genes
deoxymupirocin F + NADPH + H+→pseudomonate C + NADP+ (EC 1.1.1.M51)
No genes
mupirocin C1 + NADPH + H+→mupirocin F1 + NADP+ (EC 1.1.1.M50)
No genes
mupirocin F1 + NADPH + H+→pseudomonate A + NADP+ (EC 1.1.1.M51)
No genes

Links:

Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information