MetaCyc Pathway: superpathway of sterol biosynthesis in diatoms in Rhodosporidium toruloides IFO0880

Add experiment(s):


cycloartenol + 6 a ferrocytochrome b5 + 3 dioxygen + 5 H+→(3β,9β)-4α-demethyl-4α-carboxy-9,19-cyclolanost-24-en-3-ol + 6 a ferricytochrome b5 + 4 H2O (EC 1.14.18.10)
No genes
Sterol 24-C-methyltransferase:
cycloartenol + S-adenosyl-L-methionine→24-methylenecycloartanol + S-adenosyl-L-homocysteine + H+
(EC 2.1.1.41)
mRNA_4692 (13060)
24-methylenecycloartanol + 6 a ferrocytochrome b5 + 3 dioxygen + 5 H+→4α-carboxy-4β,14α-dimethyl-9β,19-cyclo-5α-ergost-24(241)-en-3β-ol + 6 a ferricytochrome b5 + 4 H2O (EC 1.14.18.10)
No genes
(3β,9β)-4α-demethyl-4α-carboxy-9,19-cyclolanost-24-en-3-ol + NAD+→31-norcycloartenone + CO2 + NADH (EC 1.1.1.418)
No genes
4α-carboxy-4β,14α-dimethyl-9β,19-cyclo-5α-ergost-24(241)-en-3β-ol + NAD+→cycloeucalenone + CO2 + NADH (EC 1.1.1.418)
No genes
3-keto-steroid reductase (in reverse):
31-norcycloartenone + NADPH + H+→31-norcycloartenol + NADP+
(EC 1.1.1.270)
No genes
Cycloeucalenol cycloisomerase:
31-norcycloartenol→4α,14α-dimethyl-5α-cholesta-8,24-dien-3β-ol
(EC 5.5.1.9)
No genes
3-keto-steroid reductase (in reverse):
cycloeucalenone + NADPH + H+→cycloeucalenol + NADP+
(EC 1.1.1.270)
No genes
Cycloeucalenol cycloisomerase:
cycloeucalenol→obtusifoliol
(EC 5.5.1.9)
No genes
4α,14α-dimethyl-5α-cholesta-8,24-dien-3β-ol + 3 dioxygen + 3 a reduced [NADPH-hemoprotein reductase]→4α-methyl-5α-cholesta-8,14,24-trien-3β-ol + formate + 3 an oxidized [NADPH-hemoprotein reductase] + H+ + 4 H2O (EC 1.14.14.154)
No genes
obtusifoliol + 3 dioxygen + 3 a reduced [NADPH-hemoprotein reductase]→4α-methyl-5α-ergosta-8,14,24(28)-trien-3β-ol + formate + 3 an oxidized [NADPH-hemoprotein reductase] + H+ + 4 H2O (EC 1.14.14.154)
No genes
Delta(14)-sterol reductase (in reverse):
4α-methyl-5α-cholesta-8,14,24-trien-3β-ol + NADPH + H+→4α-methyl-zymosterol + NADP+
(EC 1.3.1.70)
No genes
4α-methyl-zymosterol + 6 a ferrocytochrome b5 + 3 dioxygen + 5 H+→4α-carboxy-5α-cholesta-8,24-dien-3β-ol + 6 a ferricytochrome b5 + 4 H2O (EC 1.14.18.9)
mRNA_8272 (16640)
Delta(14)-sterol reductase (in reverse):
4α-methyl-5α-ergosta-8,14,24(28)-trien-3β-ol + NADPH + H+→4α-methylfecosterol + NADP+
(EC 1.3.1.70)
No genes
Cholestenol Delta-isomerase (in reverse):
4α-methylfecosterol→24-methylidenelophenol
(EC 5.3.3.5)
No genes
Sterol-4-alpha-carboxylate 3-dehydrogenase (decarboxylating):
4α-carboxy-5α-cholesta-8,24-dien-3β-ol + NAD(P)+→zymosterone + CO2 + NAD(P)H
(EC 1.1.1.170)
No genes
24-methylidenelophenol + 6 a ferrocytochrome b5 + 3 dioxygen + 5 H+→4α-carboxy-ergosta-7,24(241)-dien-3β-ol + 6 a ferricytochrome b5 + 4 H2O (EC 1.14.18.11)
No genes
24-methylidenelophenol + S-adenosyl-L-methionine→(24E)-24-ethylidenelophenol + S-adenosyl-L-homocysteine + H+ (EC 2.1.1.M86)
No genes
3-keto-steroid reductase (in reverse):
zymosterone + NADPH + H+→zymosterol + NADP+
(EC 1.1.1.270)
No genes
4α-carboxy-ergosta-7,24(241)-dien-3β-ol + NAD+→episterone + CO2 + NADH (EC 1.1.1.418)
No genes
(24E)-24-ethylidenelophenol + 6 a ferrocytochrome b5 + 3 dioxygen + 5 H+→(24E)-4α-carboxy-stigmasta-7,24(241)-dien-3β-ol + 6 a ferricytochrome b5 + 4 H2O (EC 1.14.18.11)
No genes
Cholestenol Delta-isomerase (in reverse):
zymosterol→5α-cholesta-7,24-dien-3β-ol
(EC 5.3.3.5)
No genes
5α-cholesta-7,24-dien-3β-ol + 2 a ferrocytochrome b5 + dioxygen + 2 H+→7-dehydrodesmosterol + 2 a ferricytochrome b5 + 2 H2O (EC 1.14.19.20)
No genes
episterone + NADPH + H+→episterol + NADP+
No genes
(24E)-4α-carboxy-stigmasta-7,24(241)-dien-3β-ol + NAD+→isoavenastenone + CO2 + NADH (EC 1.1.1.418)
No genes
3-keto-steroid reductase (in reverse):
isoavenastenone + NADPH + H+→isoavenasterol + NADP+
(EC 1.1.1.270)
No genes
episterol + 2 a ferrocytochrome b5 + dioxygen + 2 H+→ergosta-5,7,24(28)-trien-3β-ol + 2 a ferricytochrome b5 + 2 H2O (EC 1.14.19.20)
No genes
7-dehydrocholesterol reductase (in reverse):
7-dehydrodesmosterol + NADPH + H+→desmosterol + NADP+
(EC 1.3.1.21)
No genes
isoavenasterol + 2 a ferrocytochrome b5 + dioxygen + 2 H+→5-dehydroisoavenasterol + 2 a ferricytochrome b5 + 2 H2O (EC 1.14.19.20)
No genes
7-dehydrocholesterol reductase (in reverse):
ergosta-5,7,24(28)-trien-3β-ol + NADPH + H+→24-methylenecholesterol + NADP+
(EC 1.3.1.21)
No genes
Delta(24)-sterol reductase (in reverse):
desmosterol + NADPH + H+→cholesterol + NADP+
(EC 1.3.1.72)
No genes
7-dehydrocholesterol reductase (in reverse):
5-dehydroisoavenasterol + NADPH + H+→fucosterol + NADP+
(EC 1.3.1.21)
No genes
Delta(24(24(1)))-sterol reductase (in reverse):
24-methylenecholesterol + NADPH + H+→24-epi-campesterol + NADP+
(EC 1.3.1.71)
mRNA_2613 (10981)
Delta(24(24(1)))-sterol reductase (in reverse):
fucosterol + NADPH + H+→clionasterol + NADP+
(EC 1.3.1.71)
mRNA_2613 (10981)

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Candidate genes for each reaction are identified from best hits to MetaCyc; by matching EC numbers (which are assigned by TIGRFam, SEED, or best hits to KEGG); or by matching SEED roles to KEGG reactions to MetaCyc reactions. See the "Protein" tab of each gene for more information