Strain Fitness in Rhodosporidium toruloides IFO0880 around mRNA_2024

Experiment: YNB Glucose 75mM Methionine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertions' strand. Conventionally, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

500 nt 10391 and 10392 are separated by 173 nucleotides10392 and 10393 are separated by 390 nucleotides mRNA_2023: 10391 - K04718 SPHK sphingosine kinase, at 767,575 to 770,006 10391 mRNA_2024: 10392 - K15979 SND1 staphylococcal nuclease domain-containing protein 1, at 770,180 to 773,514 10392 mRNA_2025: 10393 - SUPERFAMILY--SSF53474, at 773,905 to 776,623 10393

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Per-strain Table

Position Strand Gene Sysname Fraction YNB Glucose 75mM Methionine
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769,267 + 10391 mRNA_2023 0.70 +0.3
769,452 - 10391 mRNA_2023 0.77 -1.1
769,752 + 10391 mRNA_2023 0.90 -0.5
769,860 - -1.5
770,012 - +1.0
770,015 - +0.4
770,024 + -0.4
770,032 - -1.6
770,033 + +0.5
770,052 + +0.0
770,052 + -0.9
770,177 - +1.1
770,184 + +2.7
770,187 - +0.1
770,464 - -0.3
770,745 - 10392 mRNA_2024 0.17 -0.6
770,908 - 10392 mRNA_2024 0.22 +0.1
771,044 + 10392 mRNA_2024 0.26 -0.2
771,341 - 10392 mRNA_2024 0.35 -0.0
771,354 + 10392 mRNA_2024 0.35 +1.1
771,502 + 10392 mRNA_2024 0.40 -0.6
771,633 + 10392 mRNA_2024 0.44 -0.1
771,655 - 10392 mRNA_2024 0.44 -0.4
771,722 - 10392 mRNA_2024 0.46 -0.9
772,031 + 10392 mRNA_2024 0.56 +0.5
772,947 - 10392 mRNA_2024 0.83 -0.3
773,452 - +0.3
773,518 + -1.3
773,670 - -1.8
773,672 + +1.1
773,808 - +1.0
773,832 - +1.1
773,836 - -0.8
773,843 - +1.5
773,843 + +0.4
773,850 + +0.4
773,864 - +1.0
773,981 - +1.0
773,997 + -0.7
774,001 + +0.8
774,117 - +0.4
774,121 - +2.1
774,147 + +0.3
774,263 + 10393 mRNA_2025 0.13 -0.2
774,306 + 10393 mRNA_2025 0.15 +0.3
774,388 - 10393 mRNA_2025 0.18 -2.0
774,416 - 10393 mRNA_2025 0.19 -0.3
774,440 - 10393 mRNA_2025 0.20 -0.3
774,441 + 10393 mRNA_2025 0.20 +0.3
774,441 + 10393 mRNA_2025 0.20 +0.1

Or see this region's nucleotide sequence