Strain Fitness in Rhodosporidium toruloides IFO0880 around mRNA_2179

Experiment: YNB Glucose 75mM Methionine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertions' strand. Conventionally, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

500 nt 10547 and 10548 are separated by 634 nucleotides mRNA_2179: 10547 - K06681 LTE1 Gdp/GTP exchange factor required for growth at low temperatures, at 268,224 to 273,308 10547 mRNA_2180: 10548 - KOG0166 Karyopherin (importin) alpha, at 273,943 to 276,215 10548

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Per-strain Table

Position Strand Gene Sysname Fraction YNB Glucose 75mM Methionine
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267,372 - +3.4
267,514 - +0.8
267,523 + +0.9
267,642 - -0.3
267,682 + +0.9
267,714 + -0.9
267,777 + +1.2
267,957 - -0.9
267,965 - +0.5
267,970 + +1.5
267,973 - +2.1
267,976 + -1.2
267,990 + +0.5
268,073 - +1.5
268,307 + -0.3
268,399 - -0.4
268,421 + -0.8
268,684 - +1.4
268,766 - 10547 mRNA_2179 0.11 -0.6
268,873 - 10547 mRNA_2179 0.13 +2.0
268,922 + 10547 mRNA_2179 0.14 +0.1
268,956 + 10547 mRNA_2179 0.14 +0.2
269,052 + 10547 mRNA_2179 0.16 -1.3
269,436 - 10547 mRNA_2179 0.24 +1.2
269,682 - 10547 mRNA_2179 0.29 -1.2
269,710 - 10547 mRNA_2179 0.29 -0.1
269,727 - 10547 mRNA_2179 0.30 -1.4
270,192 - 10547 mRNA_2179 0.39 +0.5
270,345 + 10547 mRNA_2179 0.42 +1.9
270,432 + 10547 mRNA_2179 0.43 +1.3
271,142 - 10547 mRNA_2179 0.57 -0.0
271,241 - 10547 mRNA_2179 0.59 +1.2
271,242 + 10547 mRNA_2179 0.59 -0.4
271,986 - 10547 mRNA_2179 0.74 -0.1
272,150 + 10547 mRNA_2179 0.77 +0.0
272,414 + 10547 mRNA_2179 0.82 -1.2
272,818 - -0.6
273,226 - +1.0
273,232 - -0.8
273,263 - +1.0
273,374 - +0.3
273,402 + -0.2
273,529 - +2.2
273,531 - -1.2
273,542 + -0.8
273,560 + +0.9
273,565 - -0.4
273,566 + +1.3
273,846 + +0.8
274,173 - 10548 mRNA_2180 0.10 -1.4
274,231 - 10548 mRNA_2180 0.13 +0.9

Or see this region's nucleotide sequence