Strain Fitness in Rhodosporidium toruloides IFO0880 around mRNA_6374

Experiment: YNB Glucose 75mM Methionine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertions' strand. Conventionally, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

500 nt 14741 and 14742 are separated by 378 nucleotides14742 and 14743 are separated by 290 nucleotides mRNA_6373: 14741 - K03013 RPB5, POLR2E DNA-directed RNA polymerases I, II, and III subunit RPABC1, at 662,336 to 663,388 14741 mRNA_6374: 14742 - K20464 OSBPL5_8, ORP5_8 oxysterol-binding protein-related protein 5/8, at 663,767 to 666,324 14742 mRNA_6375: 14743 - K01178 E3.2.1.3 glucoamylase, at 666,615 to 668,807 14743

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Per-strain Table

Position Strand Gene Sysname Fraction YNB Glucose 75mM Methionine
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663,576 + +0.3
663,798 - +0.4
664,174 + 14742 mRNA_6374 0.16 +0.7
664,749 - 14742 mRNA_6374 0.38 -0.1
665,042 - 14742 mRNA_6374 0.50 +1.2
665,332 - 14742 mRNA_6374 0.61 -0.3
665,443 - 14742 mRNA_6374 0.66 +0.0
665,473 - 14742 mRNA_6374 0.67 +0.4
665,763 + 14742 mRNA_6374 0.78 -1.1
665,891 - 14742 mRNA_6374 0.83 +0.9
665,899 - 14742 mRNA_6374 0.83 -0.3
665,900 - 14742 mRNA_6374 0.83 -0.7
665,910 + 14742 mRNA_6374 0.84 -1.0
665,929 - 14742 mRNA_6374 0.85 +0.3
665,939 + 14742 mRNA_6374 0.85 -0.3
666,026 - 14742 mRNA_6374 0.88 -0.1
666,042 + 14742 mRNA_6374 0.89 -0.2
666,042 + 14742 mRNA_6374 0.89 +1.2
666,045 - 14742 mRNA_6374 0.89 +0.4
666,057 - 14742 mRNA_6374 0.90 +0.5
666,068 + 14742 mRNA_6374 0.90 +0.5
666,069 + -0.0
666,070 - +2.1
666,070 - +1.3
666,072 + +1.6
666,075 + +0.9
666,174 + -0.2
666,203 - -0.1
666,204 + -0.7
666,205 + +1.2
666,208 - +1.7
666,211 - +0.4
666,215 - +0.1
666,220 + +0.3
666,243 - -0.6
666,341 - +0.1
666,349 - +0.6
666,356 + -2.4
666,392 + +0.7
666,666 - +0.2
666,979 + 14743 mRNA_6375 0.17 +0.7
667,258 - 14743 mRNA_6375 0.29 -0.8
667,264 - 14743 mRNA_6375 0.30 -3.5

Or see this region's nucleotide sequence