Strain Fitness in Rhodosporidium toruloides IFO0880 around mRNA_1947

Experiment: 40 hrs No Buoyancy Separation

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertions' strand. Conventionally, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

500 nt 10314 and 10315 are separated by 264 nucleotides mRNA_1946: 10314 - KOG2458 Endoplasmic reticulum protein EP58, contains filamin rod domain and KDEL motif, at 575,124 to 577,080 10314 mRNA_1947: 10315 - K14864 FTSJ1, TRM7 tRNA (cytidine32/guanosine34-2'-O)-methyltransferase, at 577,345 to 578,684 10315

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Per-strain Table

Position Strand Gene Sysname Fraction 40 hrs No Buoyancy Separation
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576,687 + 10314 mRNA_1946 0.80 -1.0
576,826 - 10314 mRNA_1946 0.87 -0.1
576,969 + -0.4
576,995 + +1.0
577,010 + -0.3
577,038 - +0.5
577,122 - +0.5
577,176 + +1.6
577,437 + +0.9
577,444 + -0.7
577,548 - 10315 mRNA_1947 0.15 -1.9
577,836 - 10315 mRNA_1947 0.37 +0.6
577,838 + 10315 mRNA_1947 0.37 +0.7
577,851 - 10315 mRNA_1947 0.38 -0.2
577,987 - 10315 mRNA_1947 0.48 -0.5
578,429 - 10315 mRNA_1947 0.81 +0.8
578,583 + +0.1
578,731 + +0.4
578,747 + +0.2
578,751 + -1.3
578,760 - -2.0
578,780 + -0.2
578,903 - +0.8
579,117 - +0.3
579,452 - -0.6
579,452 - +0.5
579,482 + +0.3
579,539 - -1.2
579,593 + -2.0
579,609 + +0.6
579,661 + -0.6

Or see this region's nucleotide sequence