Strain Fitness in Rhodosporidium toruloides IFO0880 around mRNA_2019

Experiment: 40 hrs No Buoyancy Separation

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertions' strand. Conventionally, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

500 nt 10386 and 10387 are separated by 280 nucleotides10387 and 10388 are separated by 146 nucleotides mRNA_2018: 10386 - K09554 CDC37 cell division cycle protein 37, at 758,563 to 760,344 10386 mRNA_2019: 10387 - K07088 K07088 uncharacterized protein, at 760,625 to 762,571 10387 mRNA_2020: 10388 - KOG1703 Adaptor protein Enigma and related PDZ-LIM proteins, at 762,718 to 765,022 10388

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Per-strain Table

Position Strand Gene Sysname Fraction 40 hrs No Buoyancy Separation
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760,213 + -1.5
760,363 - -1.0
760,379 + -0.2
760,382 - +2.1
760,472 - +1.6
760,504 - -0.6
760,514 - +0.6
760,966 + 10387 mRNA_2019 0.18 +0.3
761,131 - 10387 mRNA_2019 0.26 +2.4
761,179 - 10387 mRNA_2019 0.28 +1.8
761,319 - 10387 mRNA_2019 0.36 +1.3
761,581 + 10387 mRNA_2019 0.49 +0.1
762,049 + 10387 mRNA_2019 0.73 -0.1
762,168 + 10387 mRNA_2019 0.79 -4.5
762,183 - 10387 mRNA_2019 0.80 +0.9
762,185 + 10387 mRNA_2019 0.80 +0.5
762,552 - +1.3
762,590 + -1.5
762,814 + +1.1
762,874 + -0.5
762,942 - +1.4
762,985 + 10388 mRNA_2020 0.12 +0.1
763,128 - 10388 mRNA_2020 0.18 +1.2
763,475 - 10388 mRNA_2020 0.33 -0.7

Or see this region's nucleotide sequence