Strain Fitness in Rhodosporidium toruloides IFO0880 around mRNA_2778

Experiment: 40 hrs No Buoyancy Separation

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertions' strand. Conventionally, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

500 nt 11146 and 11147 are separated by 129 nucleotides mRNA_2778: 11146 - K15264 NSUN5, WBSCR20 putative methyltransferase, at 188,984 to 191,295 11146 mRNA_2779: 11147 - K18278 THI5 pyrimidine precursor biosynthesis enzyme, at 191,425 to 193,018 11147

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Per-strain Table

Position Strand Gene Sysname Fraction 40 hrs No Buoyancy Separation
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188,093 + +0.0
188,523 + -0.3
188,527 + +0.0
188,528 - +0.4
188,649 + -0.4
188,797 + -0.0
188,809 + -0.3
188,975 + +0.6
189,217 - 11146 mRNA_2778 0.10 +0.3
189,420 + 11146 mRNA_2778 0.19 -1.5
189,437 - 11146 mRNA_2778 0.20 +0.0
189,551 + 11146 mRNA_2778 0.25 +0.1
189,786 + 11146 mRNA_2778 0.35 +0.5
189,843 - 11146 mRNA_2778 0.37 +1.2
189,974 - 11146 mRNA_2778 0.43 -0.6
189,997 + 11146 mRNA_2778 0.44 +0.4
190,735 + 11146 mRNA_2778 0.76 -1.2
190,941 - 11146 mRNA_2778 0.85 -0.2
190,942 + 11146 mRNA_2778 0.85 +0.3
190,964 - 11146 mRNA_2778 0.86 +0.0
190,970 + 11146 mRNA_2778 0.86 -0.9
191,153 - +2.4
191,153 + -0.6
191,161 + -1.0
191,319 + -0.5
191,320 - -1.0
191,348 - -0.4
191,430 - -0.6
191,441 - +0.6
191,552 + +0.5
191,580 - +0.7
191,763 + 11147 mRNA_2779 0.21 -0.9
191,794 + 11147 mRNA_2779 0.23 +2.1
192,023 - 11147 mRNA_2779 0.38 +0.0
192,129 + 11147 mRNA_2779 0.44 -1.2

Or see this region's nucleotide sequence