Strain Fitness in Rhodosporidium toruloides IFO0880 around mRNA_5257

Experiment: 40 hrs No Buoyancy Separation

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertions' strand. Conventionally, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

500 nt 13625 and 13626 are separated by 199 nucleotides mRNA_5257: 13625 - K11887 PAAF1, RPN14 proteasomal ATPase-associated factor 1, at 295,850 to 297,525 13625 mRNA_5258: 13626 - KOG0538 Glycolate oxidase, at 297,725 to 299,742 13626

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Per-strain Table

Position Strand Gene Sysname Fraction 40 hrs No Buoyancy Separation
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294,861 + -1.4
294,974 + -1.3
295,112 + +0.7
295,273 + -1.9
295,386 + +0.9
295,812 - -0.3
295,969 + +0.3
296,638 + 13625 mRNA_5257 0.47 -1.0
296,951 - 13625 mRNA_5257 0.66 -0.2
296,975 + 13625 mRNA_5257 0.67 -0.8
297,151 + 13625 mRNA_5257 0.78 -0.3
297,423 + +0.9
297,786 + +0.0
297,834 - -0.9
297,842 + +2.3
297,899 + -0.3
298,098 - 13626 mRNA_5258 0.18 -0.3
298,128 - 13626 mRNA_5258 0.20 -0.3
298,128 - 13626 mRNA_5258 0.20 +0.1
298,239 + 13626 mRNA_5258 0.25 -1.3
298,291 + 13626 mRNA_5258 0.28 +1.7

Or see this region's nucleotide sequence