Strain Fitness in Rhodosporidium toruloides IFO0880 around mRNA_13

Experiment: 40 hrs No Buoyancy Separation

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertions' strand. Conventionally, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

500 nt 8380 and 8381 are separated by 362 nucleotides mRNA_12: 8380 - K12757 MYL12 myosin regulatory light chain 12, at 32,509 to 33,351 8380 mRNA_13: 8381 - BLAST putative transmembrane protein [Rhizoctonia solani 123E], at 33,714 to 35,045 8381

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Per-strain Table

Position Strand Gene Sysname Fraction 40 hrs No Buoyancy Separation
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32,719 + 8380 mRNA_12 0.25 +0.4
32,723 - 8380 mRNA_12 0.25 +0.2
32,952 + 8380 mRNA_12 0.53 +1.1
32,975 + 8380 mRNA_12 0.55 -1.2
33,006 + 8380 mRNA_12 0.59 +0.8
33,354 + +0.0
33,828 - +0.9
33,901 + 8381 mRNA_13 0.14 +0.3
34,328 - 8381 mRNA_13 0.46 +0.6
34,607 - 8381 mRNA_13 0.67 +1.6
34,610 + 8381 mRNA_13 0.67 +0.3
34,617 + 8381 mRNA_13 0.68 -2.8
34,748 - 8381 mRNA_13 0.78 +1.3
34,770 + 8381 mRNA_13 0.79 -1.0
34,773 + 8381 mRNA_13 0.80 -0.4
34,776 + 8381 mRNA_13 0.80 -1.7
34,891 - 8381 mRNA_13 0.88 +0.5
34,898 - 8381 mRNA_13 0.89 -0.7
34,918 - -0.1
34,921 - +0.5
34,926 + -0.2
35,026 - -0.4
35,030 - -0.7
35,033 - +0.4
35,033 - +0.9
35,063 + -0.5
35,071 - +0.1
35,074 + +0.1
35,078 + +1.5
35,084 - +0.1
35,189 - -1.3
35,209 - +0.9
35,212 - -0.6
35,215 - -4.2
35,222 - -0.9
35,223 + -0.3
35,226 - -0.2
35,233 + +0.2
35,274 + -2.8
35,338 - +0.8
35,340 - +0.5
35,351 - -0.9
35,367 + +0.4
35,370 + +1.6
35,388 + +0.4
35,511 - -0.7
35,520 - +0.3
35,541 + +2.5
35,545 + -0.2
35,658 - -0.9
35,660 - -0.5
35,824 - -0.5
35,850 + +0.2

Or see this region's nucleotide sequence