Strain Fitness in Rhodosporidium toruloides IFO0880 around mRNA_76

Experiment: 40 hrs No Buoyancy Separation

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertions' strand. Conventionally, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

500 nt 8443 and 8444 are separated by 138 nucleotides8444 and 8445 are separated by 57 nucleotides mRNA_75: 8443 - K00606 panB 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 180,761 to 182,108 8443 mRNA_76: 8444 - K18164 NDUFAF7 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 7, at 182,247 to 184,330 8444 mRNA_77: 8445 - K13121 FRA10AC1 protein FRA10AC1, at 184,388 to 185,510 8445

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Per-strain Table

Position Strand Gene Sysname Fraction 40 hrs No Buoyancy Separation
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181,658 - 8443 mRNA_75 0.67 +1.2
181,762 + 8443 mRNA_75 0.74 +0.0
181,763 - 8443 mRNA_75 0.74 -1.6
181,767 - 8443 mRNA_75 0.75 -1.4
181,788 - 8443 mRNA_75 0.76 +0.4
181,914 - 8443 mRNA_75 0.86 +0.2
181,916 + 8443 mRNA_75 0.86 +0.6
181,929 - 8443 mRNA_75 0.87 +2.2
182,051 + -2.0
182,063 - -0.2
182,067 - +0.1
182,089 + -0.4
182,214 + -2.1
184,330 + -0.0
184,412 - -0.0
185,119 - 8445 mRNA_77 0.65 -1.9
185,286 - 8445 mRNA_77 0.80 -0.6

Or see this region's nucleotide sequence