Strain Fitness in Rhodosporidium toruloides IFO0880 around mRNA_223

Experiment: 40 hrs No Buoyancy Separation

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertions' strand. Conventionally, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

500 nt 8590 and 8591 are separated by 210 nucleotides mRNA_222: 8590 - K12795 SUGT1, SGT1 suppressor of G2 allele of SKP1, at 547,787 to 548,767 8590 mRNA_223: 8591 - HMMPfam-Protein of unknown function (DUF1212)-PF06738,HMMPfam-Protein of unknown function (DUF3815)-PF12821, at 548,978 to 553,115 8591

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Per-strain Table

Position Strand Gene Sysname Fraction 40 hrs No Buoyancy Separation
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548,785 - +2.0
548,797 + -0.7
548,809 + +1.4
548,827 + -0.2
549,522 + 8591 mRNA_223 0.13 +1.0
549,685 - 8591 mRNA_223 0.17 -0.4
549,754 + 8591 mRNA_223 0.19 +0.3
549,828 + 8591 mRNA_223 0.21 -0.2
550,224 + 8591 mRNA_223 0.30 -1.0
550,308 - 8591 mRNA_223 0.32 +0.1
550,417 + 8591 mRNA_223 0.35 +0.0
550,551 - 8591 mRNA_223 0.38 +0.1
550,574 - 8591 mRNA_223 0.39 +2.9
550,905 + 8591 mRNA_223 0.47 +1.5
550,981 - 8591 mRNA_223 0.48 +0.1
551,135 - 8591 mRNA_223 0.52 +0.2
551,325 - 8591 mRNA_223 0.57 +0.1
551,581 - 8591 mRNA_223 0.63 +0.6
551,733 - 8591 mRNA_223 0.67 +1.2
552,168 - 8591 mRNA_223 0.77 -1.4
552,453 - 8591 mRNA_223 0.84 -0.0
552,765 + +0.1
552,910 + +0.7
552,950 - +1.1
553,042 - +0.2
553,211 + +1.6
553,253 + +0.3
553,271 - -1.8
553,273 - +0.2
553,862 - +1.9
553,866 + +0.0
553,869 + -0.2
553,884 + +0.0
553,884 + -0.1
553,890 + -0.8
553,960 - -0.7

Or see this region's nucleotide sequence