Strain Fitness in Rhodosporidium toruloides IFO0880 around mRNA_2023

Experiment: 40 hrs Low Buoyancy

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertions' strand. Conventionally, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

500 nt 10390 and 10391 are separated by 203 nucleotides10391 and 10392 are separated by 173 nucleotides mRNA_2022: 10390 - BLAST KLLA0F09339p [Kluyveromyces lactis], at 766,628 to 767,371 10390 mRNA_2023: 10391 - K04718 SPHK sphingosine kinase, at 767,575 to 770,006 10391 mRNA_2024: 10392 - K15979 SND1 staphylococcal nuclease domain-containing protein 1, at 770,180 to 773,514 10392

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Per-strain Table

Position Strand Gene Sysname Fraction 40 hrs Low Buoyancy
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766,710 - 10390 mRNA_2022 0.11 +0.8
766,725 - 10390 mRNA_2022 0.13 -0.2
766,731 + 10390 mRNA_2022 0.14 +2.3
766,850 + 10390 mRNA_2022 0.30 +1.8
766,988 - 10390 mRNA_2022 0.48 -0.5
767,181 - 10390 mRNA_2022 0.74 +1.2
767,274 + 10390 mRNA_2022 0.87 +0.4
767,415 - +0.5
767,538 - +0.0
767,710 - -1.1
767,753 + +2.0
768,148 + 10391 mRNA_2023 0.24 -0.2
768,576 - 10391 mRNA_2023 0.41 -0.2
768,625 + 10391 mRNA_2023 0.43 +0.7
768,750 - 10391 mRNA_2023 0.48 +0.2
769,041 - 10391 mRNA_2023 0.60 +0.5
769,116 - 10391 mRNA_2023 0.63 +1.2
769,267 + 10391 mRNA_2023 0.70 -1.3
769,452 - 10391 mRNA_2023 0.77 +1.6
769,752 + 10391 mRNA_2023 0.90 +2.1
769,860 - -1.0
770,012 - +1.2
770,015 - -1.2
770,024 + +0.5
770,032 - +1.1
770,033 + +0.9
770,052 + +0.4
770,052 + -0.5
770,177 - +0.9
770,184 + -1.8
770,187 - +0.7
770,464 - +1.2
770,745 - 10392 mRNA_2024 0.17 -0.2
770,908 - 10392 mRNA_2024 0.22 +0.5

Or see this region's nucleotide sequence