Strain Fitness in Rhodosporidium toruloides IFO0880 around mRNA_2054

Experiment: 40 hrs Median Buoyancy

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertions' strand. Conventionally, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

500 nt 10421 and 10422 are separated by 194 nucleotides10422 and 10423 are separated by 146 nucleotides mRNA_2053: 10421 - KOG2450 Aldehyde dehydrogenase, at 842,019 to 844,467 10421 mRNA_2054: 10422 - KOG1994 Predicted RNA binding protein, contains G-patch and Zn-finger domains, at 844,662 to 846,096 10422 mRNA_2055: 10423 - KOG2817 Predicted E3 ubiquitin ligase, at 846,243 to 848,116 10423

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Per-strain Table

Position Strand Gene Sysname Fraction 40 hrs Median Buoyancy
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843,684 + 10421 mRNA_2053 0.68 +2.4
843,718 - 10421 mRNA_2053 0.69 +0.9
843,831 - 10421 mRNA_2053 0.74 -2.1
843,880 - 10421 mRNA_2053 0.76 -0.0
843,892 + 10421 mRNA_2053 0.76 +0.3
843,970 + 10421 mRNA_2053 0.80 +0.7
843,995 + 10421 mRNA_2053 0.81 +0.2
843,997 - 10421 mRNA_2053 0.81 +1.3
844,178 + 10421 mRNA_2053 0.88 +0.2
844,335 - +0.2
844,411 - -1.4
844,427 + -0.7
844,585 - +1.3
844,585 - +0.7
844,594 + +0.4
844,733 - +0.4
844,737 - -0.5
844,774 + +1.7
844,794 + -2.0
844,855 + 10422 mRNA_2054 0.13 +1.7
844,990 + 10422 mRNA_2054 0.23 +0.2
845,190 + 10422 mRNA_2054 0.37 +0.0
845,735 - 10422 mRNA_2054 0.75 +1.1
845,780 - 10422 mRNA_2054 0.78 -0.1
845,825 - 10422 mRNA_2054 0.81 +0.7
845,846 - 10422 mRNA_2054 0.83 +0.4
845,914 + 10422 mRNA_2054 0.87 +0.8
846,030 - +1.3
846,080 - -0.0
846,084 + +0.1
846,190 - +0.6
846,275 + +0.7
846,368 - +0.4
846,545 - 10423 mRNA_2055 0.16 +0.2
846,545 + 10423 mRNA_2055 0.16 +3.7
846,672 + 10423 mRNA_2055 0.23 +1.0
846,935 - 10423 mRNA_2055 0.37 +2.1
846,940 + 10423 mRNA_2055 0.37 -0.3

Or see this region's nucleotide sequence