Strain Fitness in Rhodosporidium toruloides IFO0880 around mRNA_2179

Experiment: 40 hrs No Buoyancy Separation

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertions' strand. Conventionally, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

500 nt 10547 and 10548 are separated by 634 nucleotides mRNA_2179: 10547 - K06681 LTE1 Gdp/GTP exchange factor required for growth at low temperatures, at 268,224 to 273,308 10547 mRNA_2180: 10548 - KOG0166 Karyopherin (importin) alpha, at 273,943 to 276,215 10548

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Per-strain Table

Position Strand Gene Sysname Fraction 40 hrs No Buoyancy Separation
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267,372 - -1.4
267,514 - +0.4
267,523 + -1.4
267,642 - +0.1
267,682 + -3.0
267,714 + -0.2
267,777 + -1.0
267,957 - -1.7
267,965 - -1.7
267,970 + -0.4
267,973 - -2.3
267,976 + +1.0
267,990 + -3.7
268,073 - -0.9
268,307 + -1.7
268,399 - -1.0
268,421 + -1.4
268,684 - -0.4
268,766 - 10547 mRNA_2179 0.11 -2.3
268,873 - 10547 mRNA_2179 0.13 -3.1
268,922 + 10547 mRNA_2179 0.14 -2.8
268,956 + 10547 mRNA_2179 0.14 -1.2
269,052 + 10547 mRNA_2179 0.16 +0.9
269,436 - 10547 mRNA_2179 0.24 -2.1
269,682 - 10547 mRNA_2179 0.29 +0.3
269,710 - 10547 mRNA_2179 0.29 -1.2
269,727 - 10547 mRNA_2179 0.30 -1.6
270,192 - 10547 mRNA_2179 0.39 -1.8
270,345 + 10547 mRNA_2179 0.42 -2.2
270,432 + 10547 mRNA_2179 0.43 -1.4
271,142 - 10547 mRNA_2179 0.57 -3.1
271,241 - 10547 mRNA_2179 0.59 -1.0
271,242 + 10547 mRNA_2179 0.59 -2.6
271,986 - 10547 mRNA_2179 0.74 +0.1
272,150 + 10547 mRNA_2179 0.77 -1.3
272,414 + 10547 mRNA_2179 0.82 -2.9
272,818 - -1.2
273,226 - -2.3
273,232 - +0.2
273,263 - -1.9
273,374 - -1.6
273,402 + -2.6
273,529 - -4.6
273,531 - -0.1
273,542 + -1.6
273,560 + -2.9
273,565 - -0.6
273,566 + -1.8
273,846 + -0.3
274,173 - 10548 mRNA_2180 0.10 -2.9
274,231 - 10548 mRNA_2180 0.13 -1.3

Or see this region's nucleotide sequence