Strain Fitness in Rhodosporidium toruloides IFO0880 around mRNA_2778

Experiment: 40 hrs No Buoyancy Separation

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertions' strand. Conventionally, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

500 nt 11146 and 11147 are separated by 129 nucleotides mRNA_2778: 11146 - K15264 NSUN5, WBSCR20 putative methyltransferase, at 188,984 to 191,295 11146 mRNA_2779: 11147 - K18278 THI5 pyrimidine precursor biosynthesis enzyme, at 191,425 to 193,018 11147

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Per-strain Table

Position Strand Gene Sysname Fraction 40 hrs No Buoyancy Separation
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188,093 + -2.8
188,523 + -1.7
188,527 + +1.2
188,528 - -0.2
188,649 + +0.4
188,797 + -1.4
188,809 + -2.5
188,975 + -0.1
189,217 - 11146 mRNA_2778 0.10 +0.7
189,420 + 11146 mRNA_2778 0.19 +2.2
189,437 - 11146 mRNA_2778 0.20 -1.0
189,551 + 11146 mRNA_2778 0.25 +0.7
189,786 + 11146 mRNA_2778 0.35 -0.0
189,843 - 11146 mRNA_2778 0.37 -1.8
189,974 - 11146 mRNA_2778 0.43 +0.1
189,997 + 11146 mRNA_2778 0.44 +0.2
190,735 + 11146 mRNA_2778 0.76 -0.8
190,941 - 11146 mRNA_2778 0.85 -0.3
190,942 + 11146 mRNA_2778 0.85 -0.2
190,964 - 11146 mRNA_2778 0.86 -1.8
190,970 + 11146 mRNA_2778 0.86 -1.1
191,153 - -3.3
191,153 + -1.6
191,161 + -1.7
191,319 + -0.1
191,320 - -1.1
191,348 - -2.3
191,430 - -1.8
191,441 - -0.9
191,552 + +1.1
191,580 - -0.2
191,763 + 11147 mRNA_2779 0.21 -2.3
191,794 + 11147 mRNA_2779 0.23 -2.4
192,023 - 11147 mRNA_2779 0.38 +0.2
192,129 + 11147 mRNA_2779 0.44 -1.1

Or see this region's nucleotide sequence