Experiment: 40 hrs No Buoyancy Separation
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertions' strand.
Conventionally, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt
13356 and 13357 are separated by 760 nucleotides 13357 and 13358 are separated by 152 nucleotides
mRNA_4988: 13356 - KOG1014 17 beta-hydroxysteroid dehydrogenase type 3, HSD17B3, at 1,484,024 to 1,484,924
13356
mRNA_4989: 13357 - Hypothetical Protein, at 1,485,685 to 1,486,453
13357
mRNA_4990: 13358 - K00354 E1.6.99.1 NADPH2 dehydrogenase, at 1,486,606 to 1,488,181
13358
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Per-strain Table Position Strand Gene Sysname Fraction 40 hrs No Buoyancy Separation remove 1,484,745 - 13356 mRNA_4988 0.80 -2.1 1,484,759 + 13356 mRNA_4988 0.82 -1.8 1,484,818 - 13356 mRNA_4988 0.88 -3.7 1,484,892 - -4.6 1,484,898 - -1.7 1,484,900 + -0.2 1,484,939 + -0.7 1,485,006 - -2.8 1,485,046 - -0.6 1,485,055 + -0.2 1,485,055 + -0.3 1,485,057 - -0.6 1,485,058 + -1.0 1,485,063 - -1.2 1,485,068 + +2.0 1,485,071 - -2.6 1,485,078 + -1.1 1,485,120 + -0.2 1,485,120 + +0.4 1,485,132 - +0.9 1,485,133 + +0.6 1,485,157 - -3.2 1,485,345 - -0.8 1,485,376 - -1.0 1,485,391 + -1.2 1,485,509 - -1.8 1,485,533 + -3.4 1,485,539 + -1.1 1,485,635 + -2.1 1,485,799 - 13357 mRNA_4989 0.15 -1.9 1,485,812 - 13357 mRNA_4989 0.17 +0.8 1,485,815 - 13357 mRNA_4989 0.17 +0.0 1,485,828 - 13357 mRNA_4989 0.19 -0.7 1,485,953 + 13357 mRNA_4989 0.35 -3.7 1,485,974 - 13357 mRNA_4989 0.38 -1.8 1,485,979 - 13357 mRNA_4989 0.38 -2.5 1,486,114 + 13357 mRNA_4989 0.56 -1.7 1,486,983 + 13358 mRNA_4990 0.24 -2.2 1,487,081 + 13358 mRNA_4990 0.30 -0.2 1,487,105 + 13358 mRNA_4990 0.32 -0.7 1,487,137 + 13358 mRNA_4990 0.34 +0.3 1,487,267 + 13358 mRNA_4990 0.42 -2.2 1,487,270 - 13358 mRNA_4990 0.42 -2.8 1,487,286 + 13358 mRNA_4990 0.43 -2.0
Or see this region's nucleotide sequence