Strain Fitness in Rhodosporidium toruloides IFO0880 around mRNA_5255

Experiment: 40 hrs No Buoyancy Separation

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertions' strand. Conventionally, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

500 nt 13622 and 13623 are separated by 289 nucleotides mRNA_5254: 13622 - HMMPfam-10 TM Acyl Transferase domain found in Cas1p-PF07779,HMMPfam-GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p-PF13839, at 280,539 to 283,748 13622 mRNA_5255: 13623 - K12604 CNOT1, NOT1 CCR4-NOT transcription complex subunit 1, at 284,038 to 290,339 13623

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Per-strain Table

Position Strand Gene Sysname Fraction 40 hrs No Buoyancy Separation
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283,403 + 13622 mRNA_5254 0.89 -1.2
283,680 + -1.8
283,815 - -2.0
283,825 - -1.8
283,826 - -1.1
283,829 + -2.2
283,850 - -0.3
283,855 + -0.3
283,856 - +0.5
283,856 - -0.8
283,857 - -1.3
283,967 - -2.1
283,977 + -2.0
284,133 + -0.6
288,283 + 13623 mRNA_5255 0.67 +1.0
289,960 + -1.4
290,149 - -0.8
290,395 - +0.6
290,411 + -0.5
290,538 - -2.3
290,558 + -1.9
290,842 + +0.2
290,982 - -0.5
290,982 - -1.7

Or see this region's nucleotide sequence