Strain Fitness in Rhodosporidium toruloides IFO0880 around mRNA_13

Experiment: 40 hrs No Buoyancy Separation

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertions' strand. Conventionally, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

500 nt 8380 and 8381 are separated by 362 nucleotides mRNA_12: 8380 - K12757 MYL12 myosin regulatory light chain 12, at 32,509 to 33,351 8380 mRNA_13: 8381 - BLAST putative transmembrane protein [Rhizoctonia solani 123E], at 33,714 to 35,045 8381

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Per-strain Table

Position Strand Gene Sysname Fraction 40 hrs No Buoyancy Separation
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32,719 + 8380 mRNA_12 0.25 +1.0
32,723 - 8380 mRNA_12 0.25 -2.3
32,952 + 8380 mRNA_12 0.53 -2.8
32,975 + 8380 mRNA_12 0.55 -0.2
33,006 + 8380 mRNA_12 0.59 -1.9
33,354 + -1.1
33,828 - -2.2
33,901 + 8381 mRNA_13 0.14 -0.8
34,328 - 8381 mRNA_13 0.46 +0.3
34,607 - 8381 mRNA_13 0.67 -0.8
34,610 + 8381 mRNA_13 0.67 -0.4
34,617 + 8381 mRNA_13 0.68 -0.6
34,748 - 8381 mRNA_13 0.78 -1.6
34,770 + 8381 mRNA_13 0.79 -0.1
34,773 + 8381 mRNA_13 0.80 -2.4
34,776 + 8381 mRNA_13 0.80 -3.1
34,891 - 8381 mRNA_13 0.88 -3.1
34,898 - 8381 mRNA_13 0.89 -1.0
34,918 - -2.3
34,921 - -0.8
34,926 + -2.4
35,026 - -1.9
35,030 - -1.6
35,033 - +1.5
35,033 - +0.4
35,063 + -0.5
35,071 - -2.5
35,074 + -2.0
35,078 + +0.1
35,084 - -1.2
35,189 - -2.2
35,209 - +0.9
35,212 - -0.4
35,215 - -1.6
35,222 - -0.7
35,223 + -2.7
35,226 - -0.1
35,233 + -2.9
35,274 + -1.1
35,338 - -1.0
35,340 - -1.8
35,351 - -3.3
35,367 + -1.2
35,370 + -2.4
35,388 + -0.2
35,511 - -0.9
35,520 - -2.5
35,541 + -2.1
35,545 + -1.3
35,658 - -0.1
35,660 - -1.2
35,824 - -1.8
35,850 + -0.7

Or see this region's nucleotide sequence